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    The manuscript proposes an efficient method to gene modify cord blood-derived CD8+ T cells using lentiviral vectors and a transduction enhancer (LentiBOOST). Future application of these findings can improve the generation of allogeneic T cell therapies for the treatment of cancer.

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    The present invention relates to skin care compositions, including cosmeceuticals, for topical application, and more particularly, a skin cream, comprising exosomes and cell culture medium conditioned by cells grown in two-dimensional culture. Also included are methods of making and using such compositions and kits comprising the skin cream therein.

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    2018-5-12u2002·u2002Umbilical cord blood (UCB) CD34+ cells make up less than 0.5% of all mononucleated cells and even less in peripheral blood and bone marrow 11. To circumvent the problem of low CD34+ cell numbers in a UCB unit, we developed a method to proliferate these cells from frozen units 12, 13 .

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    2021-5-5u2002·u2002To address possible roles of BMI1 in human erythroblast proliferation, we performed an analysis of the BMI1 gene expression during the course of the erythroblast culture established from PBMCs. As shown in Figure 2A, The BMI1 mRNA was expressed significantly lower in late-stage (day 32) than in early-stage erythroblasts (days 8–16). This is consistent with the BMI1 gene expression pattern ...

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    2021-6-11u2002·u2002Umbilical cord blood (CB)-derived T cells are of particular interest, as they show a naive phenotype with elevated proliferation potential and low expression of exhaustion markers compared to PB-derived T cells. 21, 22 Additionally, Hiwarkar et al. 23 showed that CB CD8 + T cells have an increased killing effect on human leukocyte antigen (HLA ...

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    2018-12-13u2002·u2002Anin vitrofeeder-free cell culture based on Notch ligand DL-4 has been developed in our laboratory leading to the generation of T-cells progenitors from both human cord blood and adult CD34 + hematopoietic stem and progenitor cells (HSPCs) 1,2. Since TNFα is constitutively synthesized in the thymus, we explored its role during human early T ...

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    An HPLC method was developed to determine the various carotenoids in human serum. A C-30 column and a mobile phase of 100% methanol (A) and 100% methylene chloride (B) with the following gradient ...

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  • Efficient lentiviral transduction method to gene modify ...

    The manuscript proposes an efficient method to gene modify cord blood-derived CD8+ T cells using lentiviral vectors and a transduction enhancer (LentiBOOST). Future application of these findings can improve the generation of allogeneic T cell therapies for the treatment of cancer.

    Get Price
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    The present invention relates to skin care compositions, including cosmeceuticals, for topical application, and more particularly, a skin cream, comprising exosomes and cell culture medium conditioned by cells grown in two-dimensional culture. Also included are methods of making and using such compositions and kits comprising the skin cream therein.

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  • STEMCELL Technologies

    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

    Get Price
  • Topical Application of Culture‐Expanded CD34+ Umbilical ...

    2018-5-12u2002·u2002Umbilical cord blood (UCB) CD34+ cells make up less than 0.5% of all mononucleated cells and even less in peripheral blood and bone marrow 11. To circumvent the problem of low CD34+ cell numbers in a UCB unit, we developed a method to proliferate these cells from frozen units 12, 13 .

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  • BMI1 enables extensive expansion of functional ...

    2021-5-5u2002·u2002To address possible roles of BMI1 in human erythroblast proliferation, we performed an analysis of the BMI1 gene expression during the course of the erythroblast culture established from PBMCs. As shown in Figure 2A, The BMI1 mRNA was expressed significantly lower in late-stage (day 32) than in early-stage erythroblasts (days 8–16). This is consistent with the BMI1 gene expression …

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  • Plant stem cells and their applications: special emphasis ...

    2020-6-5u2002·u2002Plant stem cells are innately undifferentiated cells present in the meristematic tissues, providing them vitality and a steady supply of precursor cells which later differentiate into various parts or tissues (Batygina 2011).The two vital sources of stem cells in plants are apical and lateral meristematic tissues (Dodueva et al. 2017).The characteristic features of these cells are self-renewal ...

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  • Efficient lentiviral transduction method to gene modify ...

    2021-6-11u2002·u2002Umbilical cord blood (CB)-derived T cells are of particular interest, as they show a naive phenotype with elevated proliferation potential and low expression of exhaustion markers compared to PB-derived T cells. 21, 22 Additionally, Hiwarkar et al. 23 showed that CB CD8 + T cells have an increased killing effect on human leukocyte antigen (HLA ...

    Get Price
  • Changing the Face of Modern Medicine: Stem Cell and

    2018-12-13u2002·u2002Anin vitrofeeder-free cell culture based on Notch ligand DL-4 has been developed in our laboratory leading to the generation of T-cells progenitors from both human cord blood and adult CD34 + hematopoietic stem and progenitor cells (HSPCs) 1,2. Since TNFα is constitutively synthesized in the thymus, we explored its role during human early T ...

    Get Price
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    An HPLC method was developed to determine the various carotenoids in human serum. A C-30 column and a mobile phase of 100% methanol (A) and 100% methylene chloride (B) with the following gradient ...

    Get Price
  • Amazon.com : SCBI Stem Cell Facemasks (set of 4) : Beauty

    Facial Cream - Stem Cell Moisturizer, Anti-Wrinkle Serum for Face, Neck and Under Eyes, Natural Extracts for Clear Skin, Unscented - Coy Beauty. 4.1 out of 5 stars. 16. 1 offer from $18.99. La Mav Hyaluronic Acid Hydra-Max Face Serum - Hyperpigmentation Facial Treatment - Natural Skin Vitamins and Moisturizer. 5.0 out of 5 stars.

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    2018-12-13u2002·u2002Anin vitrofeeder-free cell culture based on Notch ligand DL-4 has been developed in our laboratory leading to the generation of T-cells progenitors from both human cord blood and adult CD34 + hematopoietic stem and progenitor cells (HSPCs) 1,2. Since TNFα is constitutively synthesized in the thymus, we explored its role during human early T ...

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    The broad effects at gene expression level make PHIs such as ciclopirox olamine (CPX) attractive compounds in promoting local angiogenesis by HIF-1α stabilization [].Under normoxic conditions CPX, a lipophilic bidentate iron chelator, stabilizes HIF-1α [].Thereby, CPX prevents the degradation of HIF-1α imitating an artificial hypoxia state, which results in an increased gene expression and ...

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    The present invention relates to skin care compositions, including cosmeceuticals, for topical application, and more particularly, a skin cream, comprising exosomes and cell culture medium conditioned by cells grown in two-dimensional culture. Also included are methods of making and using such compositions and kits comprising the skin cream therein.

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  • Efficient lentiviral transduction method to gene modify ...

    The manuscript proposes an efficient method to gene modify cord blood-derived CD8+ T cells using lentiviral vectors and a transduction enhancer (LentiBOOST). Future application of these findings can improve the generation of allogeneic T cell therapies for the treatment of cancer.

    Get Price
  • US Patent Application for SKIN CREAM Patent Application ...

    The present invention relates to skin care compositions, including cosmeceuticals, for topical application, and more particularly, a skin cream, comprising exosomes and cell culture medium conditioned by cells grown in two-dimensional culture. Also included are methods of making and using such compositions and kits comprising the skin cream therein.

    Get Price
  • STEMCELL Technologies

    STEMCELL Technologies develops cell culture media, cell separation systems, instruments and other reagents for use in life sciences research.

    Get Price
  • Topical Application of Culture‐Expanded CD34+ Umbilical ...

    2018-5-12u2002·u2002Umbilical cord blood (UCB) CD34+ cells make up less than 0.5% of all mononucleated cells and even less in peripheral blood and bone marrow 11. To circumvent the problem of low CD34+ cell numbers in a UCB unit, we developed a method to proliferate these cells from frozen units 12, 13 .

    Get Price
  • BMI1 enables extensive expansion of functional ...

    2021-5-5u2002·u2002To address possible roles of BMI1 in human erythroblast proliferation, we performed an analysis of the BMI1 gene expression during the course of the erythroblast culture established from PBMCs. As shown in Figure 2A, The BMI1 mRNA was expressed significantly lower in late-stage (day 32) than in early-stage erythroblasts (days 8–16). This is consistent with the BMI1 gene expression pattern ...

    Get Price
  • Plant stem cells and their applications: special emphasis ...

    2020-6-5u2002·u2002Plant stem cells are innately undifferentiated cells present in the meristematic tissues, providing them vitality and a steady supply of precursor cells which later differentiate into various parts or tissues (Batygina 2011).The two vital sources of stem cells in plants are apical and lateral meristematic tissues (Dodueva et al. 2017).The characteristic features of these cells are self-renewal ...

    Get Price
  • Efficient lentiviral transduction method to gene modify ...

    2021-6-11u2002·u2002Umbilical cord blood (CB)-derived T cells are of particular interest, as they show a naive phenotype with elevated proliferation potential and low expression of exhaustion markers compared to PB-derived T cells. 21, 22 Additionally, Hiwarkar et al. 23 showed that CB CD8 + T cells have an increased killing effect on human leukocyte antigen (HLA ...

    Get Price
  • Changing the Face of Modern Medicine: Stem Cell and

    2018-12-13u2002·u2002Anin vitrofeeder-free cell culture based on Notch ligand DL-4 has been developed in our laboratory leading to the generation of T-cells progenitors from both human cord blood and adult CD34 + hematopoietic stem and progenitor cells (HSPCs) 1,2. Since TNFα is constitutively synthesized in the thymus, we explored its role during human early T ...

    Get Price
  • An improved HPLC method for determination of

    An HPLC method was developed to determine the various carotenoids in human serum. A C-30 column and a mobile phase of 100% methanol (A) and 100% methylene chloride (B) with the following gradient ...

    Get Price
  • Amazon.com : SCBI Stem Cell Facemasks (set of 4) : Beauty

    Facial Cream - Stem Cell Moisturizer, Anti-Wrinkle Serum for Face, Neck and Under Eyes, Natural Extracts for Clear Skin, Unscented - Coy Beauty. 4.1 out of 5 stars. 16. 1 offer from $18.99. La Mav Hyaluronic Acid Hydra-Max Face Serum - Hyperpigmentation Facial Treatment - Natural Skin Vitamins and Moisturizer. 5.0 out of 5 stars.

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    2020-6-4u2002·u2002The bad news is that many of the leading vaccine candidates for the 2019 novel coronavirus (SARS-CoV2) are being developed using fetal cell lines that were originally derived from the tissues of aborted babies in the 1970s and 80s. With more than 6.2 million reported cases so far and more than 375,000 deaths worldwide, the burden of disease ...

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  • Ciclopirox olamine promotes the angiogenic response of ...

    The broad effects at gene expression level make PHIs such as ciclopirox olamine (CPX) attractive compounds in promoting local angiogenesis by HIF-1α stabilization [].Under normoxic conditions CPX, a lipophilic bidentate iron chelator, stabilizes HIF-1α [].Thereby, CPX prevents the degradation of HIF-1α imitating an artificial hypoxia state, which results in an increased gene expression and ...

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  • Plant stem cells and their applications: special emphasis ...

    2020-6-5u2002·u2002Plant stem cells are innately undifferentiated cells present in the meristematic tissues, providing them vitality and a steady supply of precursor cells which later differentiate into various parts or tissues (Batygina 2011).The two vital sources of stem cells in plants are apical and lateral meristematic tissues (Dodueva et al. 2017).The characteristic features of these cells are self-renewal ...

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