netherlands fetal bovine liver polypeptide healthy materials factory

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  • BRL 3A

    The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for …

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  • Immunofluorescence and Immunoelectron Microsco

    2015-9-30u2002·u2002during plastic surgery, fetal human skin at an estimated gestational age of 20 weeks, mmine and human small large intestines, rat blad- ders, monkey esophagus, rat and bovine tongues, liver, mouse myocar- dium, and thymus. Tissues were frozen in liquid nitrogen and stored at

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  • Organic Anion Transporting Polypeptides Expressed in

    2013-1-11u2002·u2002Materials. The fresh frozen human tissues used in this study were provided by the Biospecimen Shared Resource of the University of Kansas Cancer Center (Kansas City, KS). Quantigene Plex 2.0 System Reagents were purchased from Panomics Inc. (Fremont, CA). Fetal bovine serum was obtained from Hyclone (Logan, UT).

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  • Prevention of Cell Growth by Suppression of Villin

    2019-8-8u2002·u2002Materials and Methods Cell Culture HepG2 cells were provided by the RIKEN BioResource Center through the National Bio-Resource project of the Ministry of Education, Culture, Sports, Science, and Technology, Japan. Cells were cultured in poly-l-lysine-coated plates in minimum essential medium supple-mented with 10% fetal bovine serum (Thermo Fisher

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  • (PDF) The effect of cow's dietary copper intake, sire breed,

    The bovine fetal liver copper concentration was approximately 3.1 mmol/g DM and was not affected by dam's age, breed, stage of pregnancy, or copper status. Age and Breed Distribution of Cows and ...

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  • Subunit Va of human and bovine cytochrome c oxidase is

    1988-9-30u2002·u2002We have isolated a full-length cDNA clone specifying the nuclear-encoded subunit Va of the human mitochondrial respiratory enzyme cytochrome c oxidase (COX; EC 1.9.3.1.). The deduced sequence of the polypeptide is 95% identical to that of the corresponding subunit of bovine COX, which makes it the most conserved polypeptide among the known bovine/human pairs of COX subunits.

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  • Non-covalent assembly of albumin nanoparticles by

    2021-1-15u2002·u2002The murine breast cancer cell line 4T1 cells were obtained from Jiangsu Institutes of Keygen Biotech. Cells were grown in RPMI-1640 medium (Gibco, Thermo Fisher Scientific Inc.) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics (100 units/mL penicillin and 100 μg/mL streptomycin) at 37 °C in 5% CO 2 atmosphere. 2.3. Animals

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  • China Earthworm Peptide Antibacterial Nutrient Solution -

    Last Login Date: Jan 14, 2021 Business Type: Manufacturer/Factory Main Products: Transfer Factor Drying Powder, Thymopeptides Drying Powder, Sheep Embryo Extract Drying Powder, Cerebrolysin Drying Powder, Dry Powder of Bovine Fetal Liver Extract, Hepatic(Liver) Extract Antitoxic Fraction, Earthworm Polypeptide Drying Powder, Snail Polypeptide Drying Powder, Bitter Gourd Polypeptide …

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  • Peptides Powder factory, Buy good quality Peptides

    Peptides Powder factory, Buy good quality Peptides Powder products from China. Best Products. ISO9001 Peptides Powder. Inquiry Now. 99% Bovine Hide Collagen Peptides. Inquiry Now. 75% Polypeptide K Peptides Powder. Inquiry Now.

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  • Polyproline Tetramer Organizing Peptides in Fetal Bovine ...

    2.1 Materials. AChE from fetal bovine serum was purified from serum pooled from multiple animals as described [] and stored at −80°C in 50% glycerol until use.The FBS AChE was 99% pure as determined by Coomassie blue staining of an SDS gel and confirmed by measurement of specific activity. α-Cyano-4-hydroxy cinnamic acid (α-CHCA, catalog number 70990) from Fluka (a member of the Sigma ...

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  • Development and characterization of a continuous cell line ...

    2019-12-8u2002·u2002This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C.

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  • Role of the Bile Acid Transporter SLC10A1 in Liver ...

    2017-8-17u2002·u2002Most transport assays were done using transfected COS1 (African green monkey kidney) cells, but some experiments were also done with JEG3 (human choriocarcinoma) cells. Cells were maintained in culture medium (DMEM/F12+glutamax, 9% fetal bovine serum, 1% penicillin/streptomycin, 100 nM Na 2 SeO 3) at 37°C and 5% CO 2. Cells were seeded in six ...

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  • A novel human organic anion transporting polypeptide ...

    2000-1-1u2002·u2002We cloned and expressed a new organic anion transporting polypeptide (OATP), termed human OATP2, (OATP-C, LST-1; symbol SLC21A6), involved in the uptake of various lipophilic anions into human liver. The cDNA encoding OATP2 comprised 2073 base pairs, corresponding to a protein of 691 amino acids, which were 44% identical to the known human OATP. An antibody directed against …

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  • Highly antibacterial polypeptide-based amphiphilic ...

    2017-8-1u2002·u2002In this study, we aim to develop a biodegradable multifunctional siRNA delivery system with excellent cellular and blood compatibility, high cancer cell transfection efficiency and antimicrobial ability ().This delivery system (PEG-b-P3/4HB-b-EPL, EHE) was synthesized based on the polymerization of PEG, P3/4HB and EPL ().Amphiphilic EHE copolymer could be self-assembly the stable core-shell ...

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  • Fermented vegetable and fruit extract (OM-X®) stimulates ...

    OA Text is an independent open-access scientific publisher showcases innovative research and ideas aimed at improving health by linking research and practice to the benefit of society. Fermented vegetable and fruit extract (OM-X®) stimulates murine gastrointestinal tract cells and RAW264.7 cells in vitro and regulates liver gene expression in vivo

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  • Prolyl 4-hydroxylase Isoenzymes I and II Have Different ...

    2021-7-8u2002·u2002Specimens from various tissues of an apparently healthy 17-week-old gestational male human fetus (Hägg et al. 1997) were available for indirect immunofluorescence studies. In addition, a 10-gestational-week-old placenta obtained from a legal abortion and one full-term placenta were available. Other samples included liver specimens from two healthy

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  • Corning

    Corning Incorporated is one of the world's leading innovators in materials science, with a nearly 170-year track record of life-changing inventions and category-defining products

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  • Prevention of Cell Growth by Suppression of Villin ...

    2019-8-8u2002·u2002Materials and Methods Cell Culture HepG2 cells were provided by the RIKEN BioResource Center through the National Bio-Resource project of the Ministry of Education, Culture, Sports, Science, and Technology, Japan. Cells were cultured in poly-l-lysine-coated plates in minimum essential medium supple-mented with 10% fetal bovine serum (Thermo Fisher

    Get Price
  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Materials and methods. Cell Factory ... 0.2% fetal bovine serum (Bioser, Argentina) was included. In the Multi-Tray production system, the same medium was used but containing 1% fetal bovine serum, and pluronic was omitted. ... the desialylated GM-CSF is rapidly eliminated from the circulation mainly by the liver, ...

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  • Nanotoxicology of an elastin-like polypeptide rapamycin ...

    2020-3-9u2002·u2002where C is the solution concentration (M), A 280 and A 350 are absorbances at 280 nm and 350 nm respectively, l is the path length (cm) and ε is the estimated molar extinction coefficient at 280 nm: 11,585 M −1 cm −1 for FSI and 1,490 M −1 cm −1 1 for ISR 48.The purity and expected molecular weight of proteins were confirmed by SDS-PAGE gel stained with Coomassie blue dye.

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  • Organic Anion Transporting Polypeptides Expressed in ...

    2013-1-11u2002·u2002Materials. The fresh frozen human tissues used in this study were provided by the Biospecimen Shared Resource of the University of Kansas Cancer Center (Kansas City, KS). Quantigene Plex 2.0 System Reagents were purchased from Panomics Inc. (Fremont, CA). Fetal bovine …

    Get Price
  • Development and characterization of a continuous cell line ...

    2019-12-8u2002·u2002This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C.

    Get Price
  • Immunofluorescence and Immunoelectron Microscopy

    2017-2-1u2002·u2002during plastic surgery, fetal human skin at an estimated gestational age of20 weeks, munne and human small and large intestines, human and rat bladxad d~rs, monkey esophagus, rat and bovine tongues, rat liver, mouse myocarxad dIUm, and thymus. Tissues were frozen in liquid nitrogen and stored at

    Get Price
  • The contribution of bovines to human health against viral ...

    2021-7-16u2002·u2002In the last 40 years, novel viruses have evolved at a much faster pace than other pathogens. Viral diseases pose a significant threat to public health around the world. Bovines have a longstanding history of significant contributions to human nutrition, agricultural, industrial purposes, medical research, drug and vaccine development, and livelihood. The life cycle, genomic structures, viral ...

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  • Optimization of the piggyBac Transposon Using mRNA

    2013-12-3u2002·u2002Integrating and expressing stably a transgene into the cellular genome remain major challenges for gene-based therapies and for bioproduction purposes. While transposon vectors mediate efficient transgene integration, expression may be limited by epigenetic silencing, and persistent transposase expression may mediate multiple transposition cycles. Here, we evaluated the delivery of the ...

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  • The effects of short‐chain fatty acids on the ...

    2019-5-20u2002·u2002The effects of short‐chain fatty acids on the transcription and secretion of apolipoprotein A‐I in human hepatocytes in vitro.

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  • The Separation of Antler Polypeptide and Its Effects on ...

    2020-10-7u2002·u2002Background . Colla Cornus Cervi (CCC) has been used as a traditional Chinese medicine in the treatment of osteoporosis and osteonecrosis of the femoral head. However, the bioavailability of CCC is seriously limited owing to its large molecular weight and complex ingredients. In the present study, antler polypeptide was separated from CCC, and the effects of antler polypeptide on rat bone ...

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  • Bioprocessing strategies for the large-scale production of ...

    2015-11-23u2002·u2002Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have been of great interest in regenerative medicine applications because of not only their differentiation potential but also their ability to secrete bioactive factors that can modulate the immune system and promote tissue repair. This potential has initiated many early-phase clinical studies for the treatment of ...

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  • (PDF) The characterization of growth factor activity in ...

    PGF was mitogenic for rabbit fetal chondrocytes and bovine corneal endothelial cells in the range of 0.015-15 ng mL-1. Heparin alone at low concentrations (0.5 microgram mL-1) was found to be ...

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  • Bioprocessing strategies for the large-scale production of ...

    2015-11-23u2002·u2002Human mesenchymal stem cells (hMSCs), also called mesenchymal stromal cells, have been of great interest in regenerative medicine applications because of not only their differentiation potential but also their ability to secrete bioactive factors that can modulate the immune system and promote tissue repair. This potential has initiated many early-phase clinical studies for the …

    Get Price
  • Fermented vegetable and fruit extract (OM-X®) stimulates ...

    OA Text is an independent open-access scientific publisher showcases innovative research and ideas aimed at improving health by linking research and practice to the benefit of society. Fermented vegetable and fruit extract (OM-X®) stimulates murine gastrointestinal tract cells and RAW264.7 cells in vitro and regulates liver gene expression in vivo

    Get Price
  • The toxic effects of alizarin red S on catalase at the ...

    2020-4-2u2002·u20022.1. Materials ARS and CAT from bovine liver were purchased from Beijing Solarbio Ltd. Na 2 HPO 4 ·12H 2 O and NaH 2 PO 4 ·2H 2 O were purchased from Tianjin Damao Chemical Reagent Factory. Phosphate buffered solution (PBS, pH 7.2–7.4) for intracellular oxidative stress analysis was from Beijing Macgene Ltd., Beijing, China.

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  • Gastrin: A Distinct Fate of Neurogenin3 Positive ...

    Pancreatic buds from embryonic day (e) 12.5 mouse embryos were cultured in DMEM supplemented with 10% fetal bovine serum, containing 100 U/ml penicillin and 100 µg/ml streptomycin, as described previously . Explants were cultured on microporous membranes (Whatman, nucleopore Track-Etch, diameter 13 mm, pore size 8.0 micron) and no medium was ...

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  • The Separation of Antler Polypeptide and Its Effects on ...

    2020-10-7u2002·u2002Background . Colla Cornus Cervi (CCC) has been used as a traditional Chinese medicine in the treatment of osteoporosis and osteonecrosis of the femoral head. However, the bioavailability of CCC is seriously limited owing to its large molecular weight and complex ingredients. In the present study, antler polypeptide was separated from CCC, and the effects of antler polypeptide …

    Get Price
  • OMIM - Online Mendelian Inheritance in Man

    2021-9-15u2002·u2002Online Mendelian Inheritance in Man (OMIM) is a comprehensive, authoritative compendium of human genes and genetic phenotypes that is freely available and updated daily. The full-text, referenced overviews in OMIM contain information on all known mendelian disorders and over 15,000 genes.

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  • Mitochondria-Targeted and Resveratrol-Loaded Dual-Function ...

    2019-6-21u2002·u2002A subcellular organelle-targeted delivery of anti-cancer drugs is a promising strategy to maximize the anti-cancer effects and minimize the adverse effects. Herein, we prepared a mitochondria-targeted drug delivery nanoplatform based on IR780 iodide (IR780) and titanium disulfide (TiS2) nanosheets. Due to the large specific surface area of TiS2 nanosheets, the …

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  • Dissecting Those New Fake Burgers - The Weston A. Price ...

    2020-3-13u2002·u2002Fetal bovine serum (FBS) made from the blood of cow fetuses is used to feed the stem cells. Arguments for lab meat capitalize on the fact that factory production of beef (and other animal foods, from pigs to fish) is an abomination; basically, modern agriculture has turned the sacred cow into a receptacle for corn—which suits the U.S. Department of Agriculture (USDA) …

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  • A Human Monoclonal Antibody against Hepatitis B Surface ...

    2015-4-29u2002·u2002We describe the production and characterization of human monoclonal antibodies (mAb) specific for the major hepatitis B virus (HBV) S protein. The mAbs, two IgG1κ and one IgG1λ, were secreted by B-cell clones obtained from peripheral blood mononuclear cells (PBMC) of one person convalescent from acute hepatitis B and one vaccinated individual. The former …

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  • N‐ and O‐linked carbohydrates and glycosylation site ...

    2004-2-9u2002·u2002Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an early acting factor essential for regulating and differentiating hematopoietic progenitor cells as well as stimulating functional activation of mature cell populations which give rise to colonies containing granulocytes, macrophages, or a combination of both cell types [[]].The mature sequence of human GM-CSF …

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  • Development and characterization of a continuous cell line ...

    2019-12-8u2002·u2002This cell line designated EL has been maintained in Leibovitz L‐15 supplemented with 10% fetal bovine serum over 72 months, and subcultured more than 90 times. The EL cell line consisted predominantly of fibroblast‐like cells, which could survive over 100 days in vitro, and could grow at 15–32°C. The optimum temperature for growth was 27°C.

    Get Price
  • Effective cellular uptake and efflux of thyroid hormone by ...

    2008-3-12u2002·u2002COS1 cells were cultured in six- or 24-well dishes (Corning, Schiphol, The Netherlands) with DMEM/F12 medium (Invitrogen), containing 9% heat-inactivated fetal bovine serum (Invitrogen) and 100 n m sodium selenite (Sigma). Cells were cultured 24 h after transfection.

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  • Interaction of rice dwarf virus outer capsid P8 protein ...

    2006-12-6u2002·u20022 Materials and methods 2.1 Cells, viruses and antibodies. Spodoptera frugiperda (Sf9) cells were grown in 10% fetal bovine serum-supplemented-TNMFH medium (Invitrogen). Recombinant baculoviruses were obtained as described by King and Possee .

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  • The effects of short-chain fatty acids on the ...

    2019-5-20u2002·u2002Recently, we observed in healthy human volunteers that amoxicillin, a broad‐spectrum antibiotic, significantly lowered serum HDL‐C concentrations.2 Amoxicillin is a member of β‐lactam antibiotics, which negatively affects bacterial growth by binding to a primary receptor, called the penicillin‐binding protein, on the bacterial membrane ...

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  • In Vitro and In Silico Strategies to Identify OATP1B1 ...

    2011-8-23u2002·u2002Purpose To establish in vitro and in silico models that predict clinical drug–drug interactions (DDIs) with the OATP1B1 (SLCO1B1) transporter. Methods The inhibitory effect of 146 drugs and drug-like compounds on OATP1B1-mediated transport was studied in HEK293 cells. A computational model was developed to predict OATP1B1 inhibition. Concentration-dependent effects were investigated for six ...

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  • Fermented vegetable and fruit extract (OM-X®) stimulates ...

    Abstract. Aim: OM-X ® comprises edible bacterium-fermented plants and is used worldwide as a dietary supplement. The study aims to determine the biological effects of OM-X ®.. Methods: OM-X ® was added to cultured lower gastrointestinal tract cells from Wistar rats; Glucagon-like peptide-1(GLP-1) was quantified by enzyme-linked immunosorbent assay. RAW264.7 cells were cultured with OM-X ...

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  • Nuclear-Cytoplasmic Interactions Affect In Utero ...

    Abstract. We generated a clone of bovine somatic cell nuclear transfer embryos using oocyte pools from defined maternal sources to study nuclear-cytoplasmic int

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  • Mitochondria-Targeted and Resveratrol-Loaded Dual

    2019-6-21u2002·u2002A subcellular organelle-targeted delivery of anti-cancer drugs is a promising strategy to maximize the anti-cancer effects and minimize the adverse effects. Herein, we prepared a mitochondria-targeted drug delivery nanoplatform based on IR780 iodide (IR780) and titanium disulfide (TiS2) nanosheets. Due to the large specific surface area of TiS2 nanosheets, the nanoplatform could highly load ...

    Get Price
  • Publications and Educational Resources

    Virginia Cooperative Extension provides a variety of educational publications and other resources related to Agriculture, Communities, Leadership, Families, Food, Health, Gardening, Natural Resources, Youth, and 4-H.

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  • The to-do list for 'clean' meat - C&EN

    2018-10-21u2002·u2002To make large quantities of clean meat, scientists first have to figure out what to feed the cells. While cows graze on pasture and munch cheap grain, cultured cells are pampered with cell culture ...

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  • The effects of short-chain fatty acids on the ...

    2019-5-20u2002·u2002Recently, we observed in healthy human volunteers that amoxicillin, a broad‐spectrum antibiotic, significantly lowered serum HDL‐C concentrations.2 Amoxicillin is a member of β‐lactam antibiotics, which negatively affects bacterial growth by binding to a primary receptor, called the penicillin‐binding protein, on the bacterial membrane ...

    Get Price
  • Fermented vegetable and fruit extract (OM-X®) stimulates ...

    Abstract. Aim: OM-X ® comprises edible bacterium-fermented plants and is used worldwide as a dietary supplement. The study aims to determine the biological effects of OM-X ®.. Methods: OM-X ® was added to cultured lower gastrointestinal tract cells from Wistar rats; Glucagon-like peptide-1(GLP-1) was quantified by enzyme-linked immunosorbent assay. RAW264.7 cells were cultured with OM-X ...

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  • High activity and low toxicity of a novel CD71-targeting ...

    2021-2-10u2002·u2002Ferritin receptor (CD71) is an example of a very attractive cancer target, since it is highly expressed in virtually all tumor types, including metastatic loci. However, this target can be considered to be inaccessible to conventional target therapies, due to its presence in many healthy tissues. Here, we describe the preclinical evaluation of a tumor proteases-activatable human ferritin (HFt ...

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  • Targeted delivery of antisense oligonucleotides to ...

    Antisense oligonucleotide (ASO) silencing of the expression of disease-associated genes is an attractive novel therapeutic approach, but treatments are limited by the ability to deliver ASOs to cells and tissues. Following systemic administration, ASOs preferentially accumulate in liver and kidney. Among the cell types refractory to ASO uptake is the pancreatic insulin-secreting β-cell.

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  • Role of the Bile Acid Transporter SLC10A1 in Liver ...

    2017-8-17u2002·u2002Hypercholesterolemia is associated with atherosclerosis and thus represents a major health risk for ischemic heart disease and stroke, the foremost causes of death in middle- and high-income countries ().Thyroid hormone (TH) plays an important role in the regulation of different steps in lipid metabolism such as lipolysis and hepatic cholesterol clearance ().

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  • Inhibitors of ubiquitin E3 ligase as potential new ...

    2017-6-2u2002·u2002Protein ubiquitylation is an important post-translational regulation, which has been shown to be necessary for life cycle progression and survival of Plasmodium falciparum. Ubiquitin is a highly conserved 76 amino acid polypeptide, which attaches covalently to target proteins through combined action of three classes of enzymes namely, the ubiquitin-activating enzyme (E1), ubiquitin-conjugating ...

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  • Guidelines for Canadian Drinking Water Quality: Guideline ...

    2021-9-15u2002·u2002The U.S. EPA has established short-term drinking water health advisories: For children less than six years old, the health advisories are 0.0003 mg/L for microcystins and 0.0007 mg/L for cylindrospermopsin. For the rest of the population, health advisories of 0.0016 mg/L for microcystins and of 0.003 mg/L for cylindrospermopsin were established.

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  • Cell plasticity in wound healing: paracrine factors of M1 ...

    2013-4-19u2002·u2002Macrophages and fibroblasts are two major players in tissue repair and fibrosis. Despite the relevance of macrophages and fibroblasts in tissue homeostasis, remarkably little is known whether macrophages are able to influence the properties of fibroblasts. Here we investigated the role of paracrine factors secreted by classically activated (M1) and alternatively activated (M2) human ...

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  • The Separation of Antler Polypeptide and Its Effects on ...

    2020-10-7u2002·u2002Background . Colla Cornus Cervi (CCC) has been used as a traditional Chinese medicine in the treatment of osteoporosis and osteonecrosis of the femoral head. However, the bioavailability of CCC is seriously limited owing to its large molecular weight and complex ingredients. In the present study, antler polypeptide was separated from CCC, and the effects of antler polypeptide on rat bone ...

    Get Price
  • Methods Matter: Standard Production Platforms for ...

    Paulk and colleagues performed an unbiased comparative characterization of rAAV manufactured by transient transfection of HEK293 or baculoviral infection of insect cells. They discovered that rAAV capsids have post-translational modifications, including glycosylation, acetylation, phosphorylation, and methylation, and these differ between platforms. Additionally, rAAV genomes are ...

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