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  • Bovine dialyzable leukocyte extract protects against LPS ...

    Bovine dialyzable leukocyte extract (bDLE) is a dialyzate of a heterogeneous mixture of low molecular weight substances released from disintegrated leukocytes of the blood or lymphoid tissue obtained from homogenized bovine spleen. bDLE is clinically effective for a broad spectrum of diseases. To determine whether bDLE improves survival and ...

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  • The research of extraction of transfer factor in bovine ...

    The research of extraction of transfer factor in bovine spleen. LIU Xiao-ying,GE Hong-hua,GENG Liang,MING Liang,ZHANG Cheng-hai (The Biological Institute,Anhui University,Hefei 230039 China) Purpose:To compare different methods of extracting TF from bovine spleen;Method:We used Lawrence-dialysis method La wrence-ult rafiltration method ...

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  • Effect of human leukocyte and other tissue dialysates on ...

    The effect of dialyzable transfer factor (TFd) on Listeria resistance was measured by survival studies and by assessing phagocytic capacity of peritoneal macrophages. Unfractionated dialysates from human leukocytes (DLE), bovine liver, porcine spleen and kidney as well as saline were injected i.p. into NMRI mice 72 h before the i.p. injection ...

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  • Transfer Factors: Identification of Conserved Sequences in ...

    2000-4-1u2002·u2002Transfer factors are small proteins that 'transfer' the ability to express cell-mediated immunity from immune donors to non-immune recipients. We developed a process for purifying specific transfer factors to apparent homogeneity. This allowed us to separate individual transfer factors from mixtures containing several transfer factors and to demonstrate the antigen-specificity of transfer …

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  • Non-specific immunostimulatory effects of transfer factor

    colostrum- and egg-derived transfer factor can positively influence immune reaction. Our results found that phagocytosis, proliferation of spleen cells, NK cell cytotoxicity, IL-2 and

    Get Price
  • TRANSFER FACTOR: Overview, Uses, Side Effects, Precautions ...

    2021-9-18u2002·u2002A transfer factor is a chemical that is taken from a human or animal that has already developed protection against a certain disease.So far, transfer factors for …

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  • Functional analysis of bovine Nramp1 and production of ...

    High quality bovine oocytes isolated from ovaries and used to perform SCNT were matured in TCM199 (Gibco, USA) medium supplemented with 2.5 μg/ml sodium pyruvate, 50 μl/ml insulin–transferrin–selenium (ITS; Gibco, USA), 0.1 IU/ml human menopausal gonadotropin (HMG; Ningbo animal hormone factory, China), 1 μg/ml estradiol (Ningbo animal ...

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  • Bovine dialyzable leukocyte extract protects against LPS ...

    2004-1-1u2002·u2002Bovine dialyzable leukocyte extract (bDLE) produced by Laboratorio de Inmunología y Virología de la Facultad de Ciencias Biológicas de la UANL (San Nicolás de los Garza, Nuevo León, México) is a low molecular weight product (10-12 kDa) from bovine spleen, dialyzed, lyophilized, and endogenous pyrogen-free as determined by a Limulus of ...

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  • IL-12 Blocks Tfh Cell Differentiation during Salmonella ...

    2019-11-26u2002·u2002STm induced high amounts of T-bet compared to controls, but only in WT- and not KO-derived T cells (Figures 7E and 7F). T-bet was required for the massive expansion of CD44 high cells observed only in infected mice (Figures 7G, S7F, and S7G) and as expected was required for IFN-γ production (Figures S7H and S7I). Thus, during STm infection, IL ...

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  • A review on transfer factor an immune modulator ...

    2013-6-1u2002·u2002Transfer factor generated using human blood are human derived, cow and mouse spleen are bovine, murine derived. Viza and his coworkers in 1974 observed that transfer factor with known antigenic specificities can be generated from LDV/7 lymphoblastoid cell line. 8 The dialyzable transfer factors have onset period as hours and able to maintain ...

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  • Characterization and Safety Profile of Transfer Factors ...

    2021-4-29u2002·u2002Keywords: transfer factor; Imuno TF; safety profile; characterization; peptides; proteomics 1. Introduction Imuno TF® is a nutritional supplement sold worldwide and composed of oligo- and polypeptides fractions from porcine spleen commonly referred to as transfer factors (TF) and with biological activity on immune regulation.

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  • Amyloid enhancing factor activity is associated with ...

    Crude amyloid enhancing factor (AEF) drastically reduces the pre-amyloid phase on passive transfer and induces amyloid deposition in the recipient mice in 48–120h. We attempted to purify AEF from murine amyloidotic liver and spleen extracts by using gel filtration, preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ion exchange chromatography and isolated a 5.5. kDa ...

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  • Recombinant bovine spleen myristoyl CoA: protein N ...

    Recently, we have isolated full length cDNA encoding bovine spleen NMT [27] the full length cDNA was cloned and expressed in E. coli, resulting in the expression of functionally active 50 kDa NMT. Using the combination of SP-Sepharose fast flow and Mono S fast protein liquid chromatography, the enzyme was purified 20-fold with a high yield.

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  • Animal Extracts Suppliers, all Quality Animal Extracts ...

    Animal Extracts, Animal Extracts Suppliers Directory - Find variety Animal Extracts Suppliers, Manufacturers, Companies from around the World at yeast extract,milk extracting machine,olive oil extraction machine, Animal Extract

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  • Mammalian myristoyl CoA: protein N-myristoyltransferase ...

    Myristoyl CoA:Protein N-myristoyltransferase (NMT) is the enzyme which catalyses the covalent transfer of myristate from myristoyl CoA to the amino-terminal glycine residue of protein substrates. Although NMT is ubiquitous in eukaryotic cells, the enzyme levels and cellular distribution vary among tissues. In this article, we describe the properties of mammalian NMT(s) with reference to ...

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  • Oral administration of bovine milk-derived extracellular ...

    2021-6-24u2002·u2002a Western blot analysis of EV-enriched protein TSG101 in milk-derived EV samples.b TEM images of RM and CM EVs.c NTA revealed major peak as …

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  • Bovine dialyzable leukocyte extract protects against LPS ...

    2004-1-1u2002·u2002Bovine dialyzable leukocyte extract (bDLE) produced by Laboratorio de Inmunología y Virología de la Facultad de Ciencias Biológicas de la UANL (San Nicolás de los Garza, Nuevo León, México) is a low molecular weight product (10-12 kDa) from bovine spleen, dialyzed, lyophilized, and endogenous pyrogen-free as determined by a Limulus of ...

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  • Large-Pore Mesoporous-Silica-Assisted synthesis of high ...

    2021-9-15u2002·u2002Under high-temperature annealing, cations diffuse across the boundaries of reactants to form a solid solution, then nucleate, crystallize, and finally form high-quality NIR-PersL materials. Meanwhile, high-temperature annealing also increases the population of lattice defects and distortions, which are closely related to the PersL intensity and ...

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  • Overview of Bovine Leukosis - Generalized Conditions ...

    Enzootic bovine leukosis is caused by BLV, an exogenous C-type oncogenic retrovirus of the BLV-human T-lymphotropic virus group. BLV has a stable genome, does not cause chronic viremia, and has no preferred site of proviral integration. Despite the lack of preferred proviral integration sites, the tumors generated by the virus in a single ...

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  • Immunobiology of Transfer Factor - 1st Edition

    1983-1-1u2002·u2002Description. Immunobiology of Transfer Factor compiles research papers presented at the Fourth International Transfer Factor Workshop, held at the Given Institute of Pathobiology in Aspen, Colorado, on October 3-6, 1982. This book focuses on the immunologic effects of transfer factor, which are supported by in vitro and in vivo experiments that ...

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  • Transfer Factor Supplement: Uses, Benefits, Side Effects ...

    2021-6-11u2002·u2002Burger DR, Klesius PH, Vandenbark AA, et al. Transfer of keyhole limpet hemocyanin dermal reactivity to man with bovine transfer factor. Cell Immunol 1979;43:192-6. View abstract. Chng HH, Shaw D, Klesius P, Saxon A. Inability of oral bovine transfer factor to eradicate cryptosporidial infection in a patient with congenital dysgammaglobulinemia.

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  • Non-specific immunostimulatory effects of transfer factor ...

    Transfer factor essentially represents small immune messenger molecules that are produced by all higher organisms and are involved in inter-cellular communication. Transfer factor was originally described as undefined immune molecules that are derived from blood or spleen cells and can cause antigen-specific cell-mediated immunity, primarily delayed hypersensitivity and the production of ...

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  • Recombinant bovine spleen myristoyl CoA: protein N ...

    Recently, we have isolated full length cDNA encoding bovine spleen NMT [27] the full length cDNA was cloned and expressed in E. coli, resulting in the expression of functionally active 50 kDa NMT. Using the combination of SP-Sepharose fast flow and Mono S fast protein liquid chromatography, the enzyme was purified 20-fold with a high yield.

    Get Price
  • CC56

    Animals were immunized with bovine mesenteric lymph node cells.Spleen cells were fused with NS1 myeloma cells.The antibody reacts with bovine B cells in peripheral blood and lymph nodes.It precipitates a 180000 dalton molecule from the surface of bovine B cells.This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in ...

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  • 3 Supporting Information for - American Chemical Society

    2021-3-12u2002·u2002Supporting Information S-4 1 Chemicals and Materials. 2 Bovine serum albumin (BSA, 66.4 kDa), holo-transferrin and apo-transferrin from bovine 3 (76 kDa) were obtained from Yeasen Biotech. Co., Ltd. (Shanghai, China); Human ubiquitin 4 (8.6 kDa), lysozyme from egg white (14.3 kDa), myoglobin from equine heart (17 kDa), 5 carbonic anhydrase from bovine erythrocytes (29 kDa), superoxide ...

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  • Purification and Characterization of a Guanine

    Purification of GEP from Rat Spleen Cytosol. Frozen rat spleen (160 g) was homogenized (Polytron; Brinkmann) in 640 ml of TENDS buffer (20 mM Tris, pH 8.0/1 mM EDTA/1 mM NaN3/1 mM dithiothreitol/0.25 M sucrose) containing pro-tease inhibitors (aprotinin, leupeptin, soybean trypsin inhibi-tor, and lima bean trypsin inhibitor, each at 1 jg/ml; 0.5 mM

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  • 2 The Importance of Zoonotic Diseases

    Suppurative meningitis and lesions of the spleen occur in most of the animals. There are three components of the anthrax toxin. Lethal factor is a zinc metalloprotease that is believed to stimulate activity of two proteins, interleukin-1 and tumor necrosis factor-alpha, that mediate the toxic death.

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  • Phenolic-enabled nanotechnology: versatile particle ...

    2021-4-7u2002·u2002Over the past decades, we have witnessed rapid advances in exploiting nanotechnology in biomedical applications including biosensing, imaging, drug delivery, and many other disease theranostic modalities. 19,20 The accessibility, versatile reactivity, and relative biocompatibility of phenolics have catalysed the broad research in nanotechnology. As an emerging field, phenolic-enabled ...

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  • Meat Industry Protein By-Products: Sources and ...

    2016-1-1u2002·u2002Quality is also ensured by rapid cooking, allowed for by adequate heat transfer during cooking, achieved through size reduction of the raw material. The cooked mixture is separated into fat, water, and protein by screening, pressing, centrifugation, solvent extraction, and drying (Auvermann et al., 2004).

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  • Bovine dialyzable leukocyte extract protects against LPS ...

    Bovine dialyzable leukocyte extract (bDLE) is a dialyzate of a heterogeneous mixture of low molecular weight substances released from disintegrated leukocytes of the blood or lymphoid tissue obtained from homogenized bovine spleen. bDLE is clinically effective for a broad spectrum of diseases. To determine whether bDLE improves survival and ...

    Get Price
  • The research of extraction of transfer factor in bovine ...

    The research of extraction of transfer factor in bovine spleen. LIU Xiao-ying,GE Hong-hua,GENG Liang,MING Liang,ZHANG Cheng-hai (The Biological Institute,Anhui University,Hefei 230039 China) Purpose:To compare different methods of extracting TF from bovine spleen;Method:We used Lawrence-dialysis method La wrence-ult rafiltration method ...

    Get Price
  • Effect of human leukocyte and other tissue dialysates on ...

    The effect of dialyzable transfer factor (TFd) on Listeria resistance was measured by survival studies and by assessing phagocytic capacity of peritoneal macrophages. Unfractionated dialysates from human leukocytes (DLE), bovine liver, porcine spleen and kidney as well as saline were injected i.p. into NMRI mice 72 h before the i.p. injection ...

    Get Price
  • Transfer Factors: Identification of Conserved Sequences in ...

    2000-4-1u2002·u2002Transfer factors are small proteins that 'transfer' the ability to express cell-mediated immunity from immune donors to non-immune recipients. We developed a process for purifying specific transfer factors to apparent homogeneity. This allowed us to separate individual transfer factors from mixtures containing several transfer factors and to demonstrate the antigen-specificity of transfer ...

    Get Price
  • Non-specific immunostimulatory effects of transfer factor

    colostrum- and egg-derived transfer factor can positively influence immune reaction. Our results found that phagocytosis, proliferation of spleen cells, NK cell cytotoxicity, IL-2 and

    Get Price
  • TRANSFER FACTOR: Overview, Uses, Side Effects,

    2021-9-18u2002·u2002A transfer factor is a chemical that is taken from a human or animal that has already developed protection against a certain disease.So far, transfer factors for various diseases have been ...

    Get Price
  • Functional analysis of bovine Nramp1 and production of ...

    High quality bovine oocytes isolated from ovaries and used to perform SCNT were matured in TCM199 (Gibco, USA) medium supplemented with 2.5 μg/ml sodium pyruvate, 50 μl/ml insulin–transferrin–selenium (ITS; Gibco, USA), 0.1 IU/ml human menopausal gonadotropin (HMG; Ningbo animal hormone factory, China), 1 μg/ml estradiol (Ningbo animal ...

    Get Price
  • Bovine dialyzable leukocyte extract protects against LPS ...

    2004-1-1u2002·u2002Bovine dialyzable leukocyte extract (bDLE) produced by Laboratorio de Inmunología y Virología de la Facultad de Ciencias Biológicas de la UANL (San Nicolás de los Garza, Nuevo León, México) is a low molecular weight product (10-12 kDa) from bovine spleen, dialyzed, lyophilized, and endogenous pyrogen-free as determined by a Limulus of ...

    Get Price
  • IL-12 Blocks Tfh Cell Differentiation during Salmonella ...

    2019-11-26u2002·u2002STm induced high amounts of T-bet compared to controls, but only in WT- and not KO-derived T cells (Figures 7E and 7F). T-bet was required for the massive expansion of CD44 high cells observed only in infected mice (Figures 7G, S7F, and S7G) and as expected was required for IFN-γ production (Figures S7H and S7I). Thus, during STm infection, IL ...

    Get Price
  • Topic: Hyper immune Bovine Colostrum as a Low-Cost, Large ...

    2021-5-20u2002·u200212 Abstract: 13 Many different strategies have been used to fight against COVID-19 pandemic as a 14 therapeutics or prophylaxis approaches. However, none of them so far have used, 15 passive immune transfer using products from immunized farm animals. Hyper 16 immune bovine colostrums (HBC) have been used against many different 17 respiratory and gastrointestinal …

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  • 10% Bovine Serum Albumin (BSA) in Iscove's MDM

    It is also suitable for the culture of mouse hematopoietic progenitor cells in serum-free conditions. Recommended for use with MethoCult™ H4100 or MethoCult™ M3134 for hematopoietic colony-forming unit (CFU) assays (at a final concentration of 1%). Contains. • Bovine serum albumin (10%) • Iscove's MDM (buffered with NaHCO₃) Subtype ...

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  • Magnetic nanosphere-guided site-specific delivery of ...

    2015-12-15u2002·u2002lung, liver, spleen, and kidneys were excised from animals and fixed with 10% formalin for microscopy examination after hematoxylin and eosin staining. Gene transfer in vivo. The successful use of a balloon catheter for local drug delivery has been demonstrated pre-viously.23-25 The angioplasty balloon coated with the

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  • HIGHLIGHTS OF PRESCRIBING INFORMATION THROMBIN

    bovine thrombin. Bleeding may result from the development of antibodies against factor V. These may cross-react with human factor V and lead to its deficiency. • Do not re-expose patients to THROMBIN-JMI if there are known or suspected antibodies to bovine thrombin and/or factor V. • Monitor patients for abnormal coagulation laboratory values,

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  • Products - HealthAlert

    Supports immune system function during times of increased demand*. Suggested Use: Two tablets per day, or as directed. Caution: This product is processed in a facility that manufactures other products containing soy, milk, egg, wheat, peanut, tree nuts, fish, and shellfish. Supplement Facts. Serving Size: 2 Tablets.

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  • Globalization and Pollution

    2017-6-6u2002·u2002The Beijing case highlights an important feature of pollution, and that is its highly political nature. Historically – facing international outrage, embarrassment, or even potentially massive compensation payouts – both corporations and nation-states have often gone to great lengths to deny, cover up, or downplay the seriousness of pollution and related industrial accidents.

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  • Molecular characterization and expression pattern of ...

    2012-12-1u2002·u2002A full-length cDNA encoding the insulin-like growth factor binding protein-3 (IGFBP-3) was cloned from the liver of common carp (Cyprinus carpio) by RT-PCR. The IGFBP-3 cDNA sequence is 1,680 bp long and has an open reading frame of 882 bp encoding a predicted polypeptide of 293 amino acid residues. The deduced amino acid sequence contains a putative …

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  • (PDF) Tom70 Is Essential for PINK1 Import into Mitochondria

    Thus, our results suggest that PINK1 is imported into mitochondria by a unique pathway that is independent of the TOM core complex but crucially depends on …

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  • Therapeutic Human Cells: Manufacture for Cell Therapy ...

    2013-8-10u2002·u2002Abstract. Human primary cells (e.g. adult stem cells) as well as differentiated cells, including those of the immune system, have been found to be therapeutically useful and free of ethical concerns. Several products have received market authorization and numerous promising clinical trials are underway.

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  • IAT: Week 1-3 Flashcards - Quizlet

    Which factors listed below most likely contributed to the introduction and emergence of West Nile virus in the United States? (choose the one best answer) Select one: a. Climate change and environmental degradation b. Increased aquaculture and interspecies transfer of an infectious agent c. Globalization (travel and trade of humans and animals) d.

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  • Additional Products - Custom Antibody Production

    Derived from bovine, our antibody beads were designed with the researcher in mind and boast a binding capacity of ranging from 450μg to 500μg IgY per ml solid bead. These high quality anti-IgY antibody beads were fashioned through cross-linking action between Protein A/G agarose beads, bovine IgG and anti-IgY antibodies.

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  • Preparation, Characterization, and Determination of ...

    Objective. The objective of this study was to prepare, characterize, and determine immunological activities of specific transfer factor (STF) specific to human sperm antigen (HSA) for the preparation of antisperm contraceptive vaccine that can be used as an immunocontraceptive. Methods. HSA-STF was prepared using the spleens of rabbits vaccinated with HSA.

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  • Transfer of Endothelial Progenitor and Bone Marrow Cells ...

    2005-9-29u2002·u2002Preparation and Labeling of Cells for Transfer. Spleen-derived cells were loaded on ficoll, after which recovered mononuclear cells were seeded on 24-well plates (10 6 per well) coated with fibronectin (Sigma) in 0.5 mL endothelial basal medium (CellSystems) supplemented with 1 μg/mL hydrocortisone, 3 μg/mL bovine brain extract, 30 μg/mL ...

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  • Characterization of murine macrophages from bone marrow ...

    2013-2-5u2002·u2002Macrophages have heterogeneous phenotypes and complex functions within both innate and adaptive immune responses. To date, most experimental studies have been performed on macrophages derived from bone marrow, spleen and peritoneum. However, differences among macrophages from these particular sources remain unclear. In this study, the features of murine macrophages from bone marrow, spleen …

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  • Splenic suppressor of cytokine signaling 3 transgene ...

    2008-11-26u2002·u2002Cytokine profile of suppressor of cytokine signaling 3 (SOCS-3)–transduced splenic antigen-presenting cells (APCs). A, DBA/1 mice were systemically injected with 3 × 10 8 focus- forming units of Ad5SOCS-3 or Ad5 luciferase 1 day after the administration of a booster injection of type II collagen. Spleens and inguinal lymph nodes (LNs) were isolated 3 days after injection.

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  • Proteins & Enzymes - Sigma-Aldrich

    Proteins & Enzymes. Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes, protein reagents, and protein kits. In addition to highly characterized enzyme tools for protein research, we an extensive portfolio of non-enzymatic proteins for cell biology, protein ...

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  • Glossary

    (1) a live uncastrated male bovine; (2) a male bovine carcass in the B or older skeletal maturity group which exhibits masculine secondary sex characteristics. Bullock an uncastrated or latently castrated male bovine. In the carcass it is difficult to determine if the animal was an intact male at the time of slaughter.

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  • Veterinary Herbal Medicine: A Systems-Based Approach

    2009-5-15u2002·u2002It can be used during chemotherapy or pneumonia, and for chronic purulent or pyodermic skin infection—at relatively high doses in all cases (in humans, 15 mL per day for serious chronic states of immune deficiency). Doses as high as 40 mL on the first day followed by 16 mL daily were used in one positive clinical trial . Astragalus, on the ...

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  • Photon Factory Activity Report 2016 - KEK

    2018-1-12u2002·u200233. Function of Amino Acid in the Nickel Oxygen Evolution Catalyst Studied by Electrochemical X-Ray Absorption Spectroscopy (Japanese) ONISHI Sho. 2014G539,2016G647. BL-9A. 38. Development of Electrochemical XAFS Measurement System using Soft X-Ray for in-situ Observation of Water Splitting Electrocatalyst (Japanese) KUROSU Hirokatsu. 2015G629.

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  • Pathogenesis of bovine brucellosis

    Bovine brucellosis is one of the most important zoonotic diseases worldwide, and is of particular significance in developing countries. The disease, which results in serious economic losses due to ...

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