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  • Evaluation on the efficacy and immunogenicity of ...

    2019-1-31u2002·u2002G 22 pPI-2.EGFP.VP7.S 100 μg100μg Intramuscular injection 300-uL pig spleen transfer factor H22 pPI-2.EGFP 100μg100μg Intramuscular injection I 22 PoRV SC201201 strain 0.2 mL 0.2 mL Intramuscular injection J 22 PEDV CV777 strain 0.2 mL 0.2 mL Intramuscular injection k 22 PBS 0.2 mL 0.2 mL Intramuscular injection

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  • BAMBANKER®・302-14681[Detail Information]

    Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells, Differentiation, 76 (9): 981-93 (2008) Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics, 140 (6 ...

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  • Evaluation on the efficacy and immunogenicity of ...

    2018-12-7u2002·u2002Porcine rotavirus (PoRV) and porcine epidemic diarrhea virus (PEDV) usually co-infect pigs in modern large-scale piggery, which both can cause severe diarrhea in newborn piglets and lead to significant economic losses to the pig industry. The VP7 protein is the main coat protein of PoRV, and the S protein is the main structural protein of PEDV, which are capable of inducing neutralizing ...

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  • Porcine CD8adim/-NKp46high NK cells are in a highly ...

    Porcine CD8αdim/-NKp46high NK cells are in a highly activated state Kerstin H Mair 1, Andrea Müllebner 2, Sabine E Essler 1, J Catharina Duvigneau 2,AnneKStorset 3, Armin Saalmüller 1

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  • ORIGINAL ARTICLE Short-Term Administrations of a ...

    Adoptive transfer experiments. At 150 days post-transplantation, spleen cells from naïve or NPI transplanted B6 mice were either left unlabeled or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) accord-ing to the manufacturer's protocol (Molecular Probes, Eugene, OR). Fifty

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  • Understanding Haemophilus parasuis infection in porcine ...

    2009-2-5u2002·u2002Additional file 2 931 transcripts that are differentially expressed in porcine spleen following Haemophilus Parasuis infection. A total of 682 (row 8–689) transcripts have been annotated based on BLASTX searches. 'FC', Fold change, gene expression level following infection compared to the control. '≥2' represents up regulation, '<1' represents down regulation. 'p-value', significance level ...

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  • Automated Ribotyping, a Rapid Typing Method for

    A4 E.rhu. 422/1E1b) P4 A 1b Porcine spleen E.rhu. NF4E1 P10 A 2 Porcine spleen A5 E.rhu. E019 P5 1b Fish E.rhu. 2017b) P43 A 19 Porcine spleen A6 E.rhu. ATCC 19414 c) P8 A 2 Pig with endocarditis E.rhu. K003 P14 2 Wild boar meat E.rhu. Godab) P27 A 8 Godwit E.rhu. Pécs 3597b) P40 A 15 Porcine tonsil E.rhu. Tanzaniab) P41 A 16 Parrot A7 E.rhu ...

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  • Choice of immunoassay to evaluate porcine cytokine levels ...

    2020-12-1u2002·u2002Porcine ELISA kits from R&D (Minneapolis, MN), and two luminex-based assays: porcine 13-plex for Millipore (Billerica, MA), and porcine 9-plex from Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA) were used. All analyses were carried out according to the manufacturer's kit instructions.

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  • Caspase-3–mediated splenic lymphocyte apoptosis in a ...

    2014-9-1u2002·u2002Results. A high degree of splenic lymphocyte apoptosis was observed in the ROSC group. Expression of Bax and activated caspase-3 was markedly increased in splenic tissue, whereas Bcl-2 was significantly decreased in the post-ROSC group compared with the sham-operated group (P < .05) at 24 and 72 hours after ROSC.The messenger RNA levels of activated caspase-3 of splenic tissue were ...

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  • Evaluation of cytokine gene expression in porcine spleen ...

    2002-10-1u2002·u2002In this study, we identified for the first time that porcine alveolar macrophages also highly express IFN-γ in responding to LPS stimulation. Although Foss et al. [22] determined the increased expression level of IL-12 in LPS-stimulated porcine alveolar macrophages through competitive PCR, the exact increase in IL-12 content of the stimulated ...

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  • Targeted delivery of extracellular vesicles in heart injury

    2021-1-1u2002·u2002Hypoxia-inducible factor as a therapeutic target for cardioprotection. Pharmacol Ther. 2012;136:69-81 101. Ong S-G, Lee WH, Huang M, Dey D, Kodo K, Sanchez-Freire V. et al. Cross talk of combined gene and cell therapy in ischemic heart disease: role of exosomal microRNA transfer. Circulation. 2014;130:S60-S9 102.

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  • Molecular Characterization of Transcriptome-wide ...

    2011-8-7u2002·u2002Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo Ping Zhou 1, Shanli Zhai 1, Xiang Zhou 1, Ping Lin 1, Tengfei Jiang 1, Xueying Hu 2, Yunbo Jiang 3, Bin Wu 3, Qingde Zhang 1, Xuewen Xu 1, Jin-ping Li 4 ...

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  • Selenium deficiency induces spleen pathological changes in ...

    2021-5-17u2002·u2002The immune system is one aspect of health that is affected by dietary selenium (Se) levels and selenoprotein expression. Spleen is an important immune organ of the body, which is directly involved in cellular immunity. However, there are limited reports on Se levels and spleen health. Therefore, this study established a Se-deficient pig model to investigate the mechanism …

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  • Evaluation of Porcine Intestinal Epitheliocytes as an In ...

    2020-8-10u2002·u2002The use of in vitro systems that allow efficient selection of probiotic candidates with immunomodulatory properties could significantly minimize the use of experimental animals. In this work, we generated an in vitro immunoassay system based on porcine intestinal epithelial (PIE) cells and dextran sodium sulfate (DSS) administration that could be useful for the selection and …

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  • Understanding Haemophilus parasuis infection in porcine ...

    2009-2-5u2002·u2002The pig is an important agricultural animal and is an excellent mammalian model for biomedical research [].The infection caused by Haemophilus parasuis (HPS) has become an increasing threat in early-weaned pigs and in pig herds of high health status. HPS is a Gram-negative bacillus that causes Glässer's disease, which is characterized by fibrinous polyserositis, …

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  • Caspase-3–mediated splenic lymphocyte apoptosis in a ...

    2014-9-1u2002·u2002Results. A high degree of splenic lymphocyte apoptosis was observed in the ROSC group. Expression of Bax and activated caspase-3 was markedly increased in splenic tissue, whereas Bcl-2 was significantly decreased in the post-ROSC group compared with the sham-operated group (P < .05) at 24 and 72 hours after ROSC.The messenger RNA levels of activated caspase-3 of splenic …

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  • A 192 bp ERV fragment insertion in the first intron of ...

    2021-8-18u2002·u2002Toll-like receptors (TLRs) play important roles in building innate immune and inducing adaptive immune responses. Associations of the TLR genes polymorphisms with disease susceptibility, which are the basis of molecular breeding for disease resistant animals, have been reported extensively. Retrotransposon insertion polymorphisms (RIPs), as a new type of …

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  • Application of an AlphaLISA method for rapid sensitive ...

    2021-5-29u2002·u2002Abstract Infection with African swine fever virus (ASFV) causes an acute and highly lethal hemorrhagic disease that has been responsible for huge economic losses in China. To exactly detect the antigen of ASFV, we established a rapid, no-wash, one-step sandwich-type immunoassay based on the amplified luminescent proximity homogenous assay-linked …

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  • Evaluation on the efficacy and immunogenicity of ...

    2019-1-31u2002·u2002G 22 pPI-2.EGFP.VP7.S 100 μg100μg Intramuscular injection 300-uL pig spleen transfer factor H22 pPI-2.EGFP 100μg100μg Intramuscular injection I 22 PoRV SC201201 strain 0.2 mL 0.2 mL Intramuscular injection J 22 PEDV CV777 strain 0.2 mL 0.2 mL Intramuscular injection k 22 PBS 0.2 mL 0.2 mL Intramuscular injection

    Get Price
  • BAMBANKER®・302-14681[Detail Information]

    Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells, Differentiation, 76 (9): 981-93 (2008) Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics, 140 (6 ...

    Get Price
  • Evaluation on the efficacy and immunogenicity of ...

    2018-12-7u2002·u2002Porcine rotavirus (PoRV) and porcine epidemic diarrhea virus (PEDV) usually co-infect pigs in modern large-scale piggery, which both can cause severe diarrhea in newborn piglets and lead to significant economic losses to the pig industry. The VP7 protein is the main coat protein of PoRV, and the S protein is the main structural protein of PEDV, which are capable of inducing …

    Get Price
  • Porcine CD8adim/-NKp46high NK cells are in a highly ...

    Porcine CD8αdim/-NKp46high NK cells are in a highly activated state Kerstin H Mair 1, Andrea Müllebner 2, Sabine E Essler 1, J Catharina Duvigneau 2,AnneKStorset 3, Armin Saalmüller 1

    Get Price
  • ORIGINAL ARTICLE Short-Term Administrations of a ...

    Adoptive transfer experiments. At 150 days post-transplantation, spleen cells from naïve or NPI transplanted B6 mice were either left unlabeled or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) accord-ing to the manufacturer's protocol …

    Get Price
  • Understanding Haemophilus parasuis infection in porcine ...

    2009-2-5u2002·u2002Additional file 2 931 transcripts that are differentially expressed in porcine spleen following Haemophilus Parasuis infection. A total of 682 (row 8–689) transcripts have been annotated based on BLASTX searches. 'FC', Fold change, gene expression level following infection compared to the control. '≥2' represents up regulation, '<1' represents down regulation. 'p …

    Get Price
  • Automated Ribotyping, a Rapid Typing Method for Analysis ...

    A4 E.rhu. 422/1E1b) P4 A 1b Porcine spleen E.rhu. NF4E1 P10 A 2 Porcine spleen A5 E.rhu. E019 P5 1b Fish E.rhu. 2017b) P43 A 19 Porcine spleen A6 E.rhu. ATCC 19414 c) P8 A 2 Pig with endocarditis E.rhu. K003 P14 2 Wild boar meat E.rhu. Godab) P27 A 8 Godwit E.rhu. Pécs 3597b) P40 A 15 Porcine tonsil E.rhu. Tanzaniab) P41 A 16 Parrot A7 E.rhu ...

    Get Price
  • Choice of immunoassay to evaluate porcine cytokine levels ...

    2020-12-1u2002·u2002Porcine ELISA kits from R&D (Minneapolis, MN), and two luminex-based assays: porcine 13-plex for Millipore (Billerica, MA), and porcine 9-plex from Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA) were used. All analyses were carried out according to the manufacturer's kit instructions.

    Get Price
  • Caspase-3–mediated splenic lymphocyte apoptosis in a ...

    2014-9-1u2002·u2002Results. A high degree of splenic lymphocyte apoptosis was observed in the ROSC group. Expression of Bax and activated caspase-3 was markedly increased in splenic tissue, whereas Bcl-2 was significantly decreased in the post-ROSC group compared with the sham-operated group (P < .05) at 24 and 72 hours after ROSC.The messenger RNA levels of activated caspase-3 of splenic …

    Get Price
  • (PDF) ZBTB38 in Porcine Alveolar Macrophages Positively ...

    Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne flavivirus endemic in the Asia-Pacific region. Maintenance of JEV in nature involves enzootic transmission by competent Culex ...

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  • Evaluation of cytokine gene expression in porcine spleen ...

    2002-10-1u2002·u2002In this study, we identified for the first time that porcine alveolar macrophages also highly express IFN-γ in responding to LPS stimulation. Although Foss et al. [22] determined the increased expression level of IL-12 in LPS-stimulated porcine alveolar macrophages through competitive PCR, the exact increase in IL-12 content of the stimulated ...

    Get Price
  • Pig production blog: swine nutrition, pig farming ...

    The German price has reached a seasonal record low this Wednesday at €1.30/kg carcass. From the time ASF emerged in Germany, the differential between the German price (carcass) and the Spanish price (live) has been very small, and on some occasions …

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  • Molecular Cloning and Expression Analysis of Porcine ...

    Molecular Cloning and Expression Analysis of Porcine ... detected in liver, lung, brain, testis, spleen, kidney, heart, muscle, lipid, and ovary. ... It contains 28 amino acids and is highly ...

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  • MicroRNA transcriptome analysis of porcine vital organ ...

    2018-1-18u2002·u2002Porcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits T-lymphocyte and macrophage immune functions; it has significantly damaged the farming industry. Although recent studies have shown that miRNAs play important roles in immune responses, the regulatory mechanisms of miRNAs during immunosuppressive virus infection remain unclear.

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  • Japanese encephalitis

    Although considered by many in the west to be a rare and exotic infection, Japanese encephalitis is numerically one of the most important causes of viral encephalitis worldwide, with an estimated 50 000 cases and 15 000 deaths annually.1 2 About one third of patients die, and half of the survivors have severe neuropshychiatric sequelae. Most of China, Southeast Asia, and the Indian ...

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  • PRT062607 Hydrochloride (P505-15 Hydrochloride)

    PRT062607 (P505-15) is a novel, highly specific, and potent orally available small-molecule inhibitor of Syk. The potency of PRT062607 against its target kinase Syk is initially tested in two different purified kinase assays. Using a FRET assay, half-maximal Syk inhibition required 6±0.2 nM (mean±S.E.M.).

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  • Antibodies, Proteins, Kits and Reagents for Life Science ...

    Abcam, the leading supplier of protein research tools to life scientists. Discover more from a range of 118,000 antibodies, kits, proteins and other reagents

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  • Attenuation of phagocytosis of xenogeneic cells by ...

    2006-9-28u2002·u2002As shown in Figure 2A, porcine cells were almost completely cleared from blood of WT mouse recipients by 2 hours, but remained detectable in CD47 KO mouse recipients 8 hours after cell transfer. We acknowledge that antipig xenoresponses by T cells, B cells, and natural killer (NK) cells may also contribute to the rejection of pig cells in the ...

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  • Comparative analysis of apoptotic changes in peripheral ...

    2014-1-6u2002·u2002Summary and experimental design. Porcine reproductive and respiratory syndrome (PRRS) was first recognized in the United States and Europe in 1987 and 1990, respectively[1–4].Highly pathogenic PRRS (HP-PRRS) first emerged in China and Vietnam in 2006, and then spread rapidly in pigs throughout Asia[5, 6].Classical PRRSV infection induces apoptosis in different organs (lungs, …

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  • JCI - Plasmacytoid dendritic cells protect against immune ...

    2019-7-2u2002·u2002In contrast, the adoptive transfer of Tregs derived from the spleen failed in protecting mice from ConA-induced acute liver injury (Supplemental Figure 9, A–C). Of note, the number of efficiently transferred pDCs in the inflamed liver was significantly higher than that of Tregs, even though the same number of cells were initially injected ...

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  • Generating Porcine Chimeras Using Inner Cell Mass Cells ...

    2013-4-23u2002·u2002Background The development and validation of stem cell therapies using induced pluripotent stem (iPS) cells can be optimized through translational research using pigs as large animal models, because pigs have the closest characteristics to humans among non-primate animals. As the recent investigations have been heading for establishment of the human iPS cells with naïve type characteristics ...

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  • Review on the transmission porcine reproductive and ...

    2016-10-28u2002·u2002Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most costly diseases affecting intensive pig production worldwide. Control of PRRS is a complex issue and involves a combination of measures including monitoring, diagnosis, biosecurity, herd management, and immunization. In spite of the numerous studies dealing with PRRS virus epidemiology, transmission of the ...

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  • Biocompare

    First discovered in 1993 and steadily developed since then, CRISPR is a simple and robust gene-editing technology that is poised to transform basic research, translational research, as well as medicine.

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  • Immune response in piglets orally immunized with ...

    2020-2-11u2002·u2002Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome, and is associated with a number of other diseases. PCV2 is widely distributed in most developed swine industries, and is a severe economic burden. With an eye to developing an effective, safe, and convenient vaccine against PCV2-associated diseases, we have constructed a recombinant Bacillus ...

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  • Extracellular production of recombinant sus scrofa trefoil ...

    Trefoil factor 3 (TFF3) is involved in cell adhesion, motility and apoptosis, regulates mucosal immunity and maintains the functional integrity of intestinal epithelia. The upregulation of TFF3 expression in the weaning rat intestine attracted our interest. The present study hypothesized that TFF3 may serve a role in preventing diarrhea in weaning piglets, which is an important consideration ...

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  • Neuron-specific beta-III Tubulin Antibody MAB1195: R&D

    A specific band was detected for beta ‑III Tubulin at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Neuron-specific beta ‑III Tubulin Monoclonal Antibody (Catalog # MAB1195). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Reconstitution Calculator.

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  • Anti-CD68 antibody [ED1] (GTX41868)

    CD68 molecule, GP110, LAMP4, SCARD1. Background. This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction ...

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  • Compensation of Disabled Organogeneses in Genetically ...

    2020-1-14u2002·u2002Porcine fetal fibroblast cells (male) carrying TALEN-induced biallelic mutations in exon 1 of PDX1 were used for somatic cell nuclear transfer (SCNT) (Figure S1A). Two types of cloned embryos were generated from two lines of nuclear donor cells with different mutation types (Figure S1B), and these cloned embryos were transferred together to a recipient gilt (Figure S1C).

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  • Xenoextracellular matrix-rosiglitazone complex-mediated ...

    2021-9-1u2002·u20021. Introduction. The ECM has been widely recommended as a substitute biomaterial in complex organs reconstruction because of its immune privilege and great potential for clinical application [1,2].Allogenic ECM-mediated organ transplantation has achieved some success both in hard and soft tissue regeneration in recent years [3,4].However, the donor shortage of ECM has sparked the utilization ...

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  • Biocompare

    First discovered in 1993 and steadily developed since then, CRISPR is a simple and robust gene-editing technology that is poised to transform basic research, translational research, as well as medicine.

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  • Diagnosis of Porcine teschovirus encephalomyelitis in the ...

    2019-8-29u2002·u2002each tissue homogenate of tonsil, spleen, and lymph nodes, as well as EDTA blood with a DNA extraction kit e following the manufacturer's instructions. DNA from each sample was eluted in 100 µl of Buffer AE and stored at –70°C until PCR tests were performed. Detection of Porcine …

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  • Pathology of US Porcine Epidemic Diarrhea Virus Strain ...

    Porcine Epidemic Diarrhea Virus Strain PC21A in Gnotobiotic Pigs Kwonil Jung, Qiuhong Wang,1 Kelly A. Scheuer, Zhongyan Lu, Yan Zhang, and Linda J. Saif1 To understand the progression of porcine epidemic di-arrhea virus infection, we inoculated gnotobiotic pigs with a newly emerged US strain, PC21A, of the virus. At 24–48

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  • Human/Mouse TNF-alpha Antibody AF-410-NA: R&D

    Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND 50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D. Reconstitution Calculator.

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  • Excess hydrogen sulfide and polysulfides production ...

    2019-10-28u2002·u2002The protein expression levels for Mpst were confirmed by standard Western blot analyses of B6 and C3H mice using both brains and splenic lymphocytes: significantly higher expression of Mpst was observed in the frontal cortex of the C3H mouse brain than in that of the B6 brain using both anti-Mpst N-terminus (Mpst-N, P = 0.03, Fig EV1A) and anti ...

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  • Prevalence of Porcine Pseudorabies Virus and Its ...

    2020-9-25u2002·u2002Tissue samples (lymph node, kidney, spleen, and brain) were determined for PRV, PRRSV, PCV2, and CSF by using commercial real-time polymerase chain reaction (RT-PCR) kits (Beijing Anheal Laboratories, Beijing China) according to the manufacturer's instructions. If the CT value ≤30, the tested sample would be considered positive.

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  • Evidence of porcine epidemic diarrhea virus (PEDV ...

    2018-1-24u2002·u2002In 2013, PED emerged for the first time in the United States (US). The porcine epidemic diarrhea virus (PEDV) spread quickly throughout North America. Infection with PEDV causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-InDel strains circulating in the US. PEDV is mainly transmitted by the fecal–oral route.

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  • Impact of aging on inflammatory and immune responses ...

    2018-8-30u2002·u2002RESEARCH ARTICLE Impact of aging on inflammatory and immune responses during elastin peptide-induced murine emphysema Alexandre Pierre,1 Flora Lemaire,1 Aïda Meghraoui-Kheddar,1 Sandra Audonnet,2 Stéphanie Héry-Huynh,1 and Richard Le Naour1,2 1EA 4683, SFR CAP-Santé, Université de Reims Champagne-Ardenne, Reims, France; and 2Plateau Technique de …

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  • Oral recombinant Lactobacillus vaccine targeting the ...

    2018-2-9u2002·u2002Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and dendritic cells (DCs) for delivering the core neutralizing epitope (COE) of PEDV spike protein was developed with M ...

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  • Codon optimization of human factor VIII cDNAs leads to ...

    2011-1-20u2002·u2002Hemophilia A is a serious bleeding disorder caused by a deficiency in, or complete absence of, the blood coagulation factor VIII (FVIII). It is the most common hereditary coagulation disorder with an incidence approaching approximately 1 in 5000 males. 1 The disorder is an attractive candidate for gene therapy because only a modest increase in FVIII plasma concentration is needed for ...

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