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  • Cell culture

    Serum, albumin, platelet lysate . For more than 80 years fetal bovine serum (FBS) and other animal-derived materials have been widely used in cell cultures for the production of vaccines or biotherapeutics. In the past there have been several approaches to replace FBS by other ingredients to avoid the use of this critical component.

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  • Sodium Tanshinone IIA Silate Exerts Microcirculation ...

    2020-10-8u2002·u2002Spinal cord microcirculation involves functioning endothelial cells at the blood spinal cord barrier (BSCB) and maintains normal functioning of spinal cord neurons, axons, and glial cells. Protection of both the function and integrity of endothelial cells as well as the prevention of BSCB disruption may be a strong strategy for the treatment of spinal cord injury (SCI) cases. Sodium Tanshinone ...

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  • Improved Bovine ICSI Outcomes by Sperm Selected after ...

    2021-9-7u2002·u2002Improved Bovine ICSI Outcomes by Sperm Selected after Combined Heparin-Glutathione Treatment Mohammad Hadi Sekhavati,1 Farshad Shadanloo,2 Morteza S. Hosseini,2 Mojtaba Tahmoorespur,1 Mohammad Reza Nasiri,1 Mahdi Hajian,2 and Mohammad H. Nasr-Esfahani2,3 Abstract

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  • Stem cell-derived kidney organoids: engineering the ...

    2019-12-5u2002·u2002Kidney organoids can be generated from human pluripotent stem cells (PSCs) using protocols that resemble the embryonic development of the kidney. The renal structures thus generated offer great potential for disease modeling, drug screening, and possibly future therapeutic application. At the same time, use of these PSC-derived organoids is hampered by lack of maturation and off-target ...

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  • Amygdalin Promotes Fracture Healing through TGF

    2020-9-7u2002·u2002endplates and to both improve microcirculation and relieve blood stasis [16, 17]. Amygdalin has been implicated in the regulation of transforming growth factor- (TGF-) β/Smad signaling [18], which has a fundamental regulatory function for bone homeostasis [19, 20]. Therefore, we hypothesized that amygdalin can promote bone fracture healing through

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  • Hypotensive Resuscitation Combined with Polydatin

    The number of binding sites of polydatin to bovine serum albumin is approximately equal to unity and the competitive experiments suggest that the binding site is probably located on site II of ...

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  • (PDF) Guidance on Good Cell Culture Practice

    Based on review by a broad range of experts and. organisations, the aim of this Guidance is to foster. consensus among all concerned with the use of cell. and tissue culture systems, in order to ...

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  • (PDF) Neurodegeneration editor's pic 2021

    04 Exosomes Derived From Pericytes Improve Microcirculation and Protect . ... at 37 C for 1.5 h and centrifugated in 20% bovine serum. ... with 10% fetal bovine serum (FBS), 100 U/ml penicillin, ...

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  • (PDF) Effect of advanced glycation end products on ...

    Effect of advanced glycation end products on oxidative stress in endothelial cells in culture: A warning on the use of cells studied in serum-free media November 2001 Diabetologia 44(10):1310-7

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  • Cell culture

    Serum, albumin, platelet lysate . For more than 80 years fetal bovine serum (FBS) and other animal-derived materials have been widely used in cell cultures for the production of vaccines or biotherapeutics. In the past there have been several approaches to replace FBS by other ingredients to avoid the use of this critical component.

    Get Price
  • Novel lipophosphonoxin-loaded polycaprolactone

    2021-9-3u2002·u2002Cells were cultured in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (streptomycin …

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  • Scientific Program - WC11 Maastricht

    2021-8-23u2002·u2002SCIENTIFIC PROGRAM The program below is subject to change. Monday 23 August 2021 – Day 1 1.45 – 3.30 PM – Plenary sessions 1.30 – 2.15 PM WC11 Virtual Opening Ceremony and Welcome Address The official opening of the WC11 Virtual Congress with speeches from the Chair of the Local Organizing Committee, President of the…

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  • (PDF) Neurodegeneration editor's pic 2021

    04 Exosomes Derived From Pericytes Improve Microcirculation and Protect . ... at 37 C for 1.5 h and centrifugated in 20% bovine serum. ... with 10% fetal bovine serum (FBS), 100 U/ml penicillin, ...

    Get Price
  • Non-viral Gene Therapy for Stargardt Disease with

    2020-1-8u2002·u2002pCMV-ABCA4 was a gift from Robert S. Molday (University of British Columbia), and it contained the full-length human ABCA4 cDNA sequence (NCBI: NM_000350.2) on a pCEP4 backbone. Cell Culture. ARPE-19 cells were cultured in DMEM and supplemented with 10% fetal bovine serum, 100 μg/mL streptomycin, and 100 U/mL penicillin (Invitrogen).

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  • QCM ECMatrix Cell Invasion Assay, 24-well (8 µm ...

    QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. - Find MSDS or …

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  • MOFs-Based Nitric Oxide Therapy for Tendon

    2020-11-11u2002·u2002Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability. The nitric oxide (NO) therapy emerges as a promising treatment for inducing the regeneration of injured tendon by angiogenesis. Here, in this study, a system that NO-loaded metal–organic frameworks (MOFs) encapsulated in polycaprolactone (PCL)/gelatin (Gel) aligned …

    Get Price
  • BioLife Solutions to Acquire Sexton Biotechnologies to ...

    2021-8-9u2002·u2002Sexton's bioproduction tools portfolio includes proprietary closed vials for cell therapy final dose packaging, human platelet lysate (HPL) media, a bio-defined replacement for fetal bovine serum ...

    Get Price
  • Advances in keratinocyte delivery in burn wound care ...

    2018-1-1u2002·u2002Keratinocytes play a vital role in especially the proliferative phase of burn wound healing leading to epithelialisation and restoration of the vascular network. For this reason and the possibility of in vitro keratinocyte culture, keratinocytes are considered an excellent candidate for cell transplantation. 3.2.

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  • Artificial Meat Production: A New Vision of the Future ...

    2020-12-26u2002·u2002Constraints in artificial meat production. Sensorial characteristics: Its sensorial characteristics (taste, color, appearance, and texture), which were different from conventional meat.; Cost: Optimization of cell culture for large-scale production can ultimately lower the cost. Public Psychological unacceptance: considered as unnatural and rejected by few people, there is also the …

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  • The Role of Food Supplementation in Microcirculation—A ...

    2021-7-2u2002·u20021.1. Microcirculation in Physiological Conditions. Microcirculation consists of a network of small blood vessels (small arteries, arterioles, capillaries, venules, and small veins) that establish a frontier with the interstitium and with lymphatic vessels, a functionally connected triad that collaborates in maintaining the homeostasis of tissues as well as of the entire cardiovascular …

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  • Novel lipophosphonoxin-loaded polycaprolactone electrospun ...

    2021-9-3u2002·u2002Cells were cultured in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (streptomycin …

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  • Scientific Program - WC11 Maastricht

    2021-8-23u2002·u2002SCIENTIFIC PROGRAM The program below is subject to change. Monday 23 August 2021 – Day 1 1.45 – 3.30 PM – Plenary sessions 1.30 – 2.15 PM WC11 Virtual Opening Ceremony and Welcome Address The official opening of the WC11 Virtual Congress with speeches from the Chair of the Local Organizing Committee, President of the…

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  • Non-viral Gene Therapy for Stargardt Disease with ECO/pRHO ...

    2020-1-8u2002·u2002pCMV-ABCA4 was a gift from Robert S. Molday (University of British Columbia), and it contained the full-length human ABCA4 cDNA sequence (NCBI: NM_000350.2) on a pCEP4 backbone. Cell Culture. ARPE-19 cells were cultured in DMEM and supplemented with 10% fetal bovine serum, 100 μg/mL streptomycin, and 100 U/mL penicillin (Invitrogen).

    Get Price
  • 1505 Original Article H subtype vascular endothelial cells ...

    microcirculation of the femoral head and activation of a repair response. The type H vessels have been proved to mediate the growth of the bone vasculature and couple angiogenesis to osteogenesis. The aim of the study is to investigate the experimental verification of H subtype vascular endothelial cells (HSVECs) in the human femoral head.

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  • Frontiers

    2020-12-11u2002·u2002Uncontrolled protein adsorption and cell binding to biomaterial surfaces may lead to degradation, implant failure, infection, and deleterious inflammatory and immune responses. The accurate characterization of biofouling is therefore crucial for the optimization of biomaterials and devices that interface with complex biological environments composed of macromolecules, …

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  • MOFs-Based Nitric Oxide Therapy for Tendon Regeneration ...

    2020-11-11u2002·u2002Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability. The nitric oxide (NO) therapy emerges as a promising treatment for inducing the regeneration of injured tendon by angiogenesis. Here, in this study, a system that NO-loaded metal–organic frameworks (MOFs) encapsulated in polycaprolactone (PCL)/gelatin (Gel) …

    Get Price
  • FDA Approved Treatment & Products For Hair (NEW 2021 ...

    2021-2-16u2002·u2002Minoxidil, first branded as Rogaine, is an over-the-counter topical medication developed to slow balding and promote regrowth. The drug was first discovered to be effective in promoting hair growth in the late 1970s or early 1980s. At this time, minoxidil was an oral drug under the brand name Loniten.

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  • Nature Republic Hyalon Active 10 Blue Capsule Serum ...

    2020-8-31u2002·u2002Nature Republic Hyalon Active 10 Blue Capsule Serum ingredients explained: Water, Glycerin, Methylpropanediol, 1,2-Hexanediol, Caprylic/Capric Triglyceride, Propanediol, Betaine, Niacinamide, Sodium Hyaluronate, Sodium Acetylated Hyaluronate, Hydroxypropyltrimonium Hyaluronate, Hydrolyzed Sodium Hyaluronate, Hydrolyzed Hyaluronic Acid, Hyaluronic Acid, …

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  • Dr. Althea Multi-Action Infusion Serum ingredients (Explained)

    2020-7-2u2002·u2002A four amino acid peptide that is claimed to improve puffy eye bags via its anti-oedema and anti-glycation effects.To back this claim up, the manufacturer created an in-vivo study with 20 volunteers using 10% of EYESERYL (that comes down to 0.01% (!) of the pure peptide) and found that by day 15, 70% of the volunteers improved, while 95% of the ...

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  • Pretreatment of Mesenchymal Stem Cells Manipulates

    2015-6-29u2002·u2002Wells were washed and the plate blocked using 10 mg/ml heat denatured bovine serum albumin (BSA) (Sigma-Aldrich) at RT for 1 hour. MSCs were labeled with 10 μM CFDA-SE to enable visualization. Subsequently, cells were pretreated with 100 μM H 2 O 2, 100 ng/ml CXCL12, 100 ng/ml TNFα, or 100 ng/ml IFNγ for one hour.

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  • Redox status in mammalian cells and stem cells during ...

    2014-1-1u2002·u2002Improving the culture conditions for various stem cells and iPS will be particularly important for clinical applications. Further research is required to establish feeder-free and serum-free defined culture conditions for the maintenance of stem and iPS cells in healthy conditions and for inducing their differentiation into desired cell types.

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  • Xenobiotic Particle Exposure and Microvascular Endpoints ...

    The observed makeup of particle aerosols is a function of the metric of interest. For the mass distribution of particles (blue line) the dominant mode is generally larger than 1 μm, whereas on a number basis (red line) the dominant mode is usually less than 0.1 μm.In between those ranges is a mode where the majority of the surface area occurs, usually around 0.2–0.3 μm.

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  • Synergistic effect of nanofat and mouse nerve‐growth ...

    2020-10-11u2002·u2002After digestion, an equal volume of Dulbecco's modified Eagle's medium (DMEM; Gibco, Carlsbad, California) supplemented with 10% fetal bovine serum (FBS; Gibco) was added and the suspension was passed through a 200 μm mesh filter followed by centrifugation at 800g for 5 minutes).

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  • (PDF) INVESTIGATION OF BOVINE HERPESVIRUS-1 (BHV-1 ...

    INVESTIGATION OF BOVINE HERPESVIRUS-1 (BHV-1) INFECTION IN RELATION TO FERTILITY IN REPEAT BREEDER COwS ISTRAŽIVANJE INFEKCIJE …

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  • FDA Approved Treatment & Products For Hair (NEW 2021 ...

    2021-2-16u2002·u2002Minoxidil, first branded as Rogaine, is an over-the-counter topical medication developed to slow balding and promote regrowth. The drug was first discovered to be effective in promoting hair growth in the late 1970s or early 1980s. At this time, minoxidil was an oral drug under the brand name Loniten.

    Get Price
  • (PDF) Effect of advanced glycation end products on ...

    Effect of advanced glycation end products on oxidative stress in endothelial cells in culture: A warning on the use of cells studied in serum-free media November 2001 Diabetologia 44(10):1310-7

    Get Price
  • BioLife Solutions to Acquire Sexton Biotechnologies to ...

    2021-8-9u2002·u2002Sexton's bioproduction tools portfolio includes proprietary closed vials for cell therapy final dose packaging, human platelet lysate (HPL) media, a bio-defined replacement for fetal bovine serum ...

    Get Price
  • Trichomonas

    T. foetus has a trophozoite stage, with a pyriform or ovoid appearance, and a size ranging from 8 to 18 μm in length and 4 to 9 μm in width (BonDurant and Honigberg 1994) (Fig. 14.1).The trophozoite has several structures with locomotor function such as flagella and the undulating membrane. The flagella originate from the basal bodies or kinetosomes—located in the apical pole of the cell.

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  • EzineArticles Submission - Submit Your Best Quality ...

    EzineArticles.com allows expert authors in hundreds of niche fields to get massive levels of exposure in exchange for the submission of their quality original articles.

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  • Journal of Ma terials Chemistry B - Royal Society of Chemistry

    following suppliers, and used as provided unless stated. Hydrogen tetrachloroaurate (III) trihydrate and trisodium citrate dibasic were from Acros Organics. Tissue culture treated multiwell plates were from BD Falcon. Deuterium oxide and methanol d-4 were from Cambridge Isotope Laboratories, Inc. Fetal bovine serum (FBS),

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  • Hypotensive Resuscitation Combined with Polydatin Improve ...

    The number of binding sites of polydatin to bovine serum albumin is approximately equal to unity and the competitive experiments suggest that the binding site is probably located on site II of ...

    Get Price
  • (PDF) Neurodegeneration editor's pic 2021

    04 Exosomes Derived From Pericytes Improve Microcirculation and Protect . ... at 37 C for 1.5 h and centrifugated in 20% bovine serum. ... with 10% fetal bovine serum (FBS), 100 U/ml penicillin, ...

    Get Price
  • Low Serum Cultured Adipose Tissue-Derived Stromal Cells ...

    2013-2-1u2002·u2002Cells were cultured under the following two conditions: a low serum culture medium containing 2% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) and 10 ng/ml human fibroblast growth factor-2 (FGF-2) (Peprotech, Inc., Rocky Hill, NJ, USA) and a high serum culture medium containing 20% FBS and 10 ng/ml human FGF-2.

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  • Trends and ideas in technology, regulation and public ...

    2021-6-1u2002·u2002The first challenge is to remove the fetal bovine serum (FBS) and other animal-derived components from the culture medium. FBS is an important and universal growth-promoting supplement in animal cell medium, but it has high price, complicated and unclear composition, large batch difference, and bring the risk of virus contamination ( Fang et al ...

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  • Scientific Program - WC11 Maastricht

    2021-8-23u2002·u2002SCIENTIFIC PROGRAM The program below is subject to change. Monday 23 August 2021 – Day 1 1.45 – 3.30 PM – Plenary sessions 1.30 – 2.15 PM WC11 Virtual Opening Ceremony and Welcome Address The official opening of the WC11 Virtual Congress with speeches from the Chair of the Local Organizing Committee, President of the…

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  • Surfaces modified with covalently-immobilized adhesive ...

    1996-1-1u2002·u2002Depletion of fibronectin from fetal bovine serum Fibronectin was removed from fetal bovine serum by passing the serum through a gelatin-Sepharose column21'22. The effectiveness of the removal process was evaluated by performing an immunoblot (Western blot) on the depleted serum using a rabbit anti-rat fibronectin antibody.

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  • Influence of surface roughness on neural differentiation ...

    Matrigel was chosen to coat the surface in current work due to its great enhancement of neural differentiation . For the source of the human iPSCs, IMR90-4 was chosen in our study. This cell line was generated from IMR90 fetal lung fibroblasts by viral transduction of a combination of Oct 4, Sox 2, Nanog, and Lin 28 genes.

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  • Stem cell-derived kidney organoids: engineering the ...

    2019-12-5u2002·u2002Kidney organoids can be generated from human pluripotent stem cells (PSCs) using protocols that resemble the embryonic development of the kidney. The renal structures thus generated offer great potential for disease modeling, drug screening, and possibly future therapeutic application. At the same time, use of these PSC-derived organoids is hampered by …

    Get Price
  • Artificial Meat Production: A New Vision of the Future ...

    2020-12-26u2002·u2002Constraints in artificial meat production. Sensorial characteristics: Its sensorial characteristics (taste, color, appearance, and texture), which were different from conventional meat.; Cost: Optimization of cell culture for large-scale production can ultimately lower the cost. Public Psychological unacceptance: considered as unnatural and rejected by few people, there is …

    Get Price
  • Cell culture

    Serum, albumin, platelet lysate . For more than 80 years fetal bovine serum (FBS) and other animal-derived materials have been widely used in cell cultures for the production of vaccines or biotherapeutics. In the past there have been several approaches to replace FBS by other ingredients to avoid the use of this critical component.

    Get Price
  • Novel lipophosphonoxin-loaded polycaprolactone

    2021-9-3u2002·u2002Cells were cultured in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (streptomycin …

    Get Price
  • Scientific Program - WC11 Maastricht

    2021-8-23u2002·u2002SCIENTIFIC PROGRAM The program below is subject to change. Monday 23 August 2021 – Day 1 1.45 – 3.30 PM – Plenary sessions 1.30 – 2.15 PM WC11 Virtual Opening Ceremony and Welcome Address The official opening of the WC11 Virtual Congress with speeches from the Chair of the Local Organizing Committee, President of the…

    Get Price
  • (PDF) Neurodegeneration editor's pic 2021

    04 Exosomes Derived From Pericytes Improve Microcirculation and Protect . ... at 37 C for 1.5 h and centrifugated in 20% bovine serum. ... with 10% fetal bovine serum (FBS), 100 U/ml penicillin, ...

    Get Price
  • Non-viral Gene Therapy for Stargardt Disease with

    2020-1-8u2002·u2002pCMV-ABCA4 was a gift from Robert S. Molday (University of British Columbia), and it contained the full-length human ABCA4 cDNA sequence (NCBI: NM_000350.2) on a pCEP4 backbone. Cell Culture. ARPE-19 cells were cultured in DMEM and supplemented with 10% fetal bovine serum, 100 μg/mL streptomycin, and 100 U/mL penicillin (Invitrogen).

    Get Price
  • QCM ECMatrix Cell Invasion Assay, 24-well (8 µm ...

    QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. - Find MSDS or …

    Get Price
  • MOFs-Based Nitric Oxide Therapy for Tendon

    2020-11-11u2002·u2002Tendon regeneration is still a great challenge due to its avascular structure and low self-renewal capability. The nitric oxide (NO) therapy emerges as a promising treatment for inducing the regeneration of injured tendon by angiogenesis. Here, in this study, a system that NO-loaded metal–organic frameworks (MOFs) encapsulated in polycaprolactone (PCL)/gelatin (Gel) aligned …

    Get Price
  • BioLife Solutions to Acquire Sexton Biotechnologies to ...

    2021-8-9u2002·u2002Sexton's bioproduction tools portfolio includes proprietary closed vials for cell therapy final dose packaging, human platelet lysate (HPL) media, a bio-defined replacement for fetal bovine serum ...

    Get Price
  • Advances in keratinocyte delivery in burn wound care ...

    2018-1-1u2002·u2002Keratinocytes play a vital role in especially the proliferative phase of burn wound healing leading to epithelialisation and restoration of the vascular network. For this reason and the possibility of in vitro keratinocyte culture, keratinocytes are considered an excellent candidate for cell transplantation. 3.2.

    Get Price
  • Artificial Meat Production: A New Vision of the Future ...

    2020-12-26u2002·u2002Constraints in artificial meat production. Sensorial characteristics: Its sensorial characteristics (taste, color, appearance, and texture), which were different from conventional meat.; Cost: Optimization of cell culture for large-scale production can ultimately lower the cost. Public Psychological unacceptance: considered as unnatural and rejected by few people, there is also the …

    Get Price