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  • Newborn calf serum

    Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture …

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  • Improving passive transfer of immunoglobulins in calves. I ...

    2009-4-1u2002·u2002Introduction. Colostrum management is the single most important management factor determining calf health and survival (McGuirk and Collins, 2004).Calves may be defined as having failure of passive transfer (FPT) of protective colostral immunoglobulins (Ig) if the serum IgG concentration is <10 mg/mL when sampled between 24 and 48 h of age (NAHMS, 1996; Weaver …

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  • A consensus introduction to serum replacements and serum ...

    2017-2-1u2002·u2002Introduction. The cell therapy industry is a fast-growing industry targeted toward myriad clinical indications. Most cell therapy companies are currently conducting clinical trials in phase 1 or 2 with several that are more mature in phase 3 or even early market approvals .As the cell therapy industry matures and clinical trials hit their pivotal phase 3 studies, there will be a significant ...

    Get Price
  • PETA urges DCGI to stop use of newborn calf serum in Covaxin

    2021-6-17u2002·u2002In a letter sent on Thursday, PETA requested DCGI Dr VG Somani to direct vaccine manufacturers to replace newborn calf serum (NBCS) with animal-free, commercially available, and chemically defined ...

    Get Price
  • PETA urges DCGI to replace calf serum method for vax ...

    'The calves used in the extraction of this serum are taken away from their mothers shortly after birth, which traumatises and distresses both the mother and calf,' says PETA India Science Policy Adviser Dr Ankita Pandey. 'PETA India looks to the Drugs Controller to ensure that vaccine manufacturers switch to available animal-free media that overcome the limitations associated …

    Get Price
  • In vitro wear testing of a contemporary design of reverse ...

    2015-9-18u2002·u2002The lubricant employed was 50% newborn calf serum (Sigma-Aldrich n4637) giving an average protein content of 26 g/l. Gravimetric measurements (Denver Instruments TB-215D, sensitivity 10 µg) were used to determine the weight change and thus the wear of components. At regular intervals the simulator was stopped, lubricant was decanted and frozen ...

    Get Price
  • Lactic acid bacteria to improve growth performance in ...

    2010-5-11u2002·u2002During calf rearing, feed cost reduction is a key issue for system sustainability. In this way, incorporation of alternative sources of protein, replacement of the natural ingredients, and/or changes in the ratio of ingredients in milk substitutes can improve profitability.

    Get Price
  • Biomedical Engineering: The Recent Trends - ScienceDirect

    2018-1-1u2002·u2002Biomedical Engineering (BME) is the combination of application of engineering principles and design concepts to biology and medicine for advanced healthcare in diagnostic, monitoring, and therapy. The word bioengineering was coined by British scientist and broadcaster Heinz Wolff in …

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  • Detection of colostrum-derived alloantibodies in calves ...

    A multi-country matched case-control study was conducted in 2011 to identify calf-level risk factors for BNP. 405 BNP cases were recruited from 330 farms in Belgium, France, Germany and the ...

    Get Price
  • A frictional study of total hip joint replacements ...

    Thus there is a wide variation in reported COF values (0.03-1) (Mischler and Munoz, 2013). Scholes et al., (2000) investigated the frictional behaviour of commercially-available total hip joint ...

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  • 42 questions with answers in ADIPOGENESIS

    2021-3-11u2002·u2002We used newborn calf serum as a supplement during propagation and never allowed cells to reach over 80% confluency. We tried corning cell culture …

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  • Flow cytometry-based method for rapid and high

    2018-4-1u2002·u2002The cells were cultured in RPMI 1640 (Sigma–Aldrich, St. Louis, MO, USA) with 10% heat-inactivated newborn calf serum (NBS: Biowest, Nuaillé, France) and recombinant mouse interleukin 6 (IL6: 1 ng/mL) purified from IL6-overexpressed Escherichia coli.

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  • Biomedical Engineering: The Recent Trends - ScienceDirect

    2018-1-1u2002·u2002Biomedical Engineering (BME) is the combination of application of engineering principles and design concepts to biology and medicine for advanced healthcare in diagnostic, monitoring, and therapy. The word bioengineering was coined by British scientist and broadcaster Heinz Wolff in 1954.

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  • Impact of volume, immunoglobulin G concentration, and

    2021-9-1u2002·u2002Calf serum samples were collected at a median of 25.5 h after birth (range: 24–36 h) with no statistically significant difference in time of blood collection among groups (P = 0.97).Mean and SD serum IgG concentrations were 35.3 ± 8.4, 39.1 ± 12.6, and 32.6 ± 5.3 g/L for SH, MM, and LH group calves, respectively, and were not noted as statistically different (P = 0.2) among groups (Fig. 2A).

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  • Cell Culture Media: A Review - labome.com

    2021-8-26u2002·u2002Presence of serum in the media has many drawbacks and can lead to serious misinterpretations in immunological studies [2, 3].A number of serum-free media have been developed [4, 5].These media are generally specifically formulated to support the culture of a single cell type, such as Knockout Serum Replacement and Knockout DMEM from Thermo Fisher Scientific, and mTESR …

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  • (PDF) Guidance on Good Cell Culture Practice

    Based on review by a broad range of experts and. organisations, the aim of this Guidance is to foster. consensus among all concerned with the use of cell. and tissue culture systems, in order to ...

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  • Serum Immunoglobulin G Concentrations in Calves Fed

    Colostrum is a yellowish milky fluid produced by the breasts in the last few weeks of pregnancy and in the first 2-3 days after childbirth. It is rich in fats, sugars, proteins, antibodies, growth ...

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  • Quality Farm & Industrial Supplies and Clothing

    QC Supply has served working men and women since 1982 offering industrial supplies, farm and livestock supplies, workwear and clothing, and so much more. QC Supply prides itself on customer satisfaction and quality products.

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  • Stephen LEBLANC, Professor, BSc(Agr), DVM, DVSc,

    This study compares serum markers for systemic inflammation, and liver, mineral, and energy status in samples obtained −7, 1, 3, 5, 7, 14 ± 1, 21 ± 1, and 35 d relative to calving from healthy ...

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  • Potential of Newborn and Adult Stem Cells for the

    2015-10-4u2002·u20022.2. Production of Tissue-Engineered Vascular Constructs. The tissue-engineered vascular constructs were produced using the self-organization technique previously described [9, 29].Briefly, cells were seeded on tissue culture flask (T75, BD Bioscience) in DME-Ham (3 : 1), supplemented with 10% FCS and 50 μg/mL ascorbic acid.After 3 weeks in culture, cell sheets were peeled off the flask and ...

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  • Newborn calf serum

    Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance ...

    Get Price
  • Improving passive transfer of immunoglobulins in calves. I ...

    2009-4-1u2002·u2002Introduction. Colostrum management is the single most important management factor determining calf health and survival (McGuirk and Collins, 2004).Calves may be defined as having failure of passive transfer (FPT) of protective colostral immunoglobulins (Ig) if the serum IgG concentration is <10 mg/mL when sampled between 24 and 48 h of age (NAHMS, 1996; Weaver et al., 2000).

    Get Price
  • A consensus introduction to serum replacements and

    2017-2-1u2002·u2002Introduction. The cell therapy industry is a fast-growing industry targeted toward myriad clinical indications. Most cell therapy companies are currently conducting clinical trials in phase 1 or 2 with several that are more mature in phase 3 or even early market approvals .As the cell therapy industry matures and clinical trials hit their pivotal phase 3 studies, there will be a significant ...

    Get Price
  • PETA urges DCGI to stop use of newborn calf serum in

    2021-6-17u2002·u2002In a letter sent on Thursday, PETA requested DCGI Dr VG Somani to direct vaccine manufacturers to replace newborn calf serum (NBCS) with animal-free, commercially available, and chemically defined ...

    Get Price
  • PETA urges DCGI to replace calf serum method for vax ...

    'The calves used in the extraction of this serum are taken away from their mothers shortly after birth, which traumatises and distresses both the mother and calf,' says PETA India Science Policy Adviser Dr Ankita Pandey. 'PETA India looks to the Drugs Controller to ensure that vaccine manufacturers switch to available animal-free media that overcome the limitations associated with the use of ...

    Get Price
  • In vitro wear testing of a contemporary design of reverse ...

    2015-9-18u2002·u2002The lubricant employed was 50% newborn calf serum (Sigma-Aldrich n4637) giving an average protein content of 26 g/l. Gravimetric measurements (Denver Instruments TB-215D, sensitivity 10 µg) were used to determine the weight change and thus the wear of components. At regular intervals the simulator was stopped, lubricant was decanted and frozen ...

    Get Price
  • Lactic acid bacteria to improve growth performance in ...

    2010-5-11u2002·u2002During calf rearing, feed cost reduction is a key issue for system sustainability. In this way, incorporation of alternative sources of protein, replacement of the natural ingredients, and/or changes in the ratio of ingredients in milk substitutes can improve profitability.

    Get Price
  • Biomedical Engineering: The Recent Trends - ScienceDirect

    2018-1-1u2002·u2002Biomedical Engineering (BME) is the combination of application of engineering principles and design concepts to biology and medicine for advanced healthcare in diagnostic, monitoring, and therapy. The word bioengineering was coined by British scientist and broadcaster Heinz Wolff in 1954.

    Get Price
  • Detection of colostrum-derived alloantibodies in calves ...

    A multi-country matched case-control study was conducted in 2011 to identify calf-level risk factors for BNP. 405 BNP cases were recruited from 330 farms in Belgium, France, Germany and the ...

    Get Price
  • A frictional study of total hip joint replacements ...

    Thus there is a wide variation in reported COF values (0.03-1) (Mischler and Munoz, 2013). Scholes et al., (2000) investigated the frictional behaviour of commercially-available total hip joint ...

    Get Price
  • Newborn calf serum

    Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance ...

    Get Price
  • Improving passive transfer of immunoglobulins in calves. I ...

    2009-4-1u2002·u2002Introduction. Colostrum management is the single most important management factor determining calf health and survival (McGuirk and Collins, 2004).Calves may be defined as having failure of passive transfer (FPT) of protective colostral immunoglobulins (Ig) if the serum IgG concentration is <10 mg/mL when sampled between 24 and 48 h of age (NAHMS, 1996; Weaver et al., 2000).

    Get Price
  • A consensus introduction to serum replacements and

    2017-2-1u2002·u2002Introduction. The cell therapy industry is a fast-growing industry targeted toward myriad clinical indications. Most cell therapy companies are currently conducting clinical trials in phase 1 or 2 with several that are more mature in phase 3 or even early market approvals .As the cell therapy industry matures and clinical trials hit their pivotal phase 3 studies, there will be a significant ...

    Get Price
  • PETA urges DCGI to stop use of newborn calf serum in

    2021-6-17u2002·u2002In a letter sent on Thursday, PETA requested DCGI Dr VG Somani to direct vaccine manufacturers to replace newborn calf serum (NBCS) with animal-free, commercially available, and chemically defined ...

    Get Price
  • PETA urges DCGI to replace calf serum method for vax ...

    'The calves used in the extraction of this serum are taken away from their mothers shortly after birth, which traumatises and distresses both the mother and calf,' says PETA India Science Policy Adviser Dr Ankita Pandey. 'PETA India looks to the Drugs Controller to ensure that vaccine manufacturers switch to available animal-free media that overcome the limitations associated with the use of ...

    Get Price
  • In vitro wear testing of a contemporary design of reverse ...

    2015-9-18u2002·u2002The lubricant employed was 50% newborn calf serum (Sigma-Aldrich n4637) giving an average protein content of 26 g/l. Gravimetric measurements (Denver Instruments TB-215D, sensitivity 10 µg) were used to determine the weight change and thus the wear of components. At regular intervals the simulator was stopped, lubricant was decanted and frozen ...

    Get Price
  • Lactic acid bacteria to improve growth performance in ...

    2010-5-11u2002·u2002During calf rearing, feed cost reduction is a key issue for system sustainability. In this way, incorporation of alternative sources of protein, replacement of the natural ingredients, and/or changes in the ratio of ingredients in milk substitutes can improve profitability.

    Get Price
  • Biomedical Engineering: The Recent Trends - ScienceDirect

    2018-1-1u2002·u2002Biomedical Engineering (BME) is the combination of application of engineering principles and design concepts to biology and medicine for advanced healthcare in diagnostic, monitoring, and therapy. The word bioengineering was coined by British scientist and broadcaster Heinz Wolff in 1954.

    Get Price
  • Detection of colostrum-derived alloantibodies in calves ...

    A multi-country matched case-control study was conducted in 2011 to identify calf-level risk factors for BNP. 405 BNP cases were recruited from 330 farms in Belgium, France, Germany and the ...

    Get Price
  • A frictional study of total hip joint replacements ...

    Thus there is a wide variation in reported COF values (0.03-1) (Mischler and Munoz, 2013). Scholes et al., (2000) investigated the frictional behaviour of commercially-available total hip joint ...

    Get Price
  • Newborn calf serum

    Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance ...

    Get Price
  • Improving passive transfer of immunoglobulins in calves. I ...

    2009-4-1u2002·u2002Introduction. Colostrum management is the single most important management factor determining calf health and survival (McGuirk and Collins, 2004).Calves may be defined as having failure of passive transfer (FPT) of protective colostral immunoglobulins (Ig) if the serum IgG concentration is <10 mg/mL when sampled between 24 and 48 h of age (NAHMS, 1996; Weaver et al., 2000).

    Get Price
  • A consensus introduction to serum replacements and

    2017-2-1u2002·u2002Introduction. The cell therapy industry is a fast-growing industry targeted toward myriad clinical indications. Most cell therapy companies are currently conducting clinical trials in phase 1 or 2 with several that are more mature in phase 3 or even early market approvals .As the cell therapy industry matures and clinical trials hit their pivotal phase 3 studies, there will be a significant ...

    Get Price
  • PETA urges DCGI to stop use of newborn calf serum in

    2021-6-17u2002·u2002In a letter sent on Thursday, PETA requested DCGI Dr VG Somani to direct vaccine manufacturers to replace newborn calf serum (NBCS) with animal-free, commercially available, and chemically defined ...

    Get Price
  • PETA urges DCGI to replace calf serum method for vax ...

    'The calves used in the extraction of this serum are taken away from their mothers shortly after birth, which traumatises and distresses both the mother and calf,' says PETA India Science Policy Adviser Dr Ankita Pandey. 'PETA India looks to the Drugs Controller to ensure that vaccine manufacturers switch to available animal-free media that overcome the limitations associated with the use of ...

    Get Price
  • In vitro wear testing of a contemporary design of reverse ...

    2015-9-18u2002·u2002The lubricant employed was 50% newborn calf serum (Sigma-Aldrich n4637) giving an average protein content of 26 g/l. Gravimetric measurements (Denver Instruments TB-215D, sensitivity 10 µg) were used to determine the weight change and thus the wear of components. At regular intervals the simulator was stopped, lubricant was decanted and frozen ...

    Get Price
  • Lactic acid bacteria to improve growth performance in ...

    2010-5-11u2002·u2002During calf rearing, feed cost reduction is a key issue for system sustainability. In this way, incorporation of alternative sources of protein, replacement of the natural ingredients, and/or changes in the ratio of ingredients in milk substitutes can improve profitability.

    Get Price
  • Biomedical Engineering: The Recent Trends - ScienceDirect

    2018-1-1u2002·u2002Biomedical Engineering (BME) is the combination of application of engineering principles and design concepts to biology and medicine for advanced healthcare in diagnostic, monitoring, and therapy. The word bioengineering was coined by British scientist and broadcaster Heinz Wolff in 1954.

    Get Price
  • Detection of colostrum-derived alloantibodies in calves ...

    A multi-country matched case-control study was conducted in 2011 to identify calf-level risk factors for BNP. 405 BNP cases were recruited from 330 farms in Belgium, France, Germany and the ...

    Get Price
  • A frictional study of total hip joint replacements ...

    Thus there is a wide variation in reported COF values (0.03-1) (Mischler and Munoz, 2013). Scholes et al., (2000) investigated the frictional behaviour of commercially-available total hip joint ...

    Get Price
  • Setd4 controlled quiescent c-Kit+ cells contribute to ...

    2021-6-2u2002·u2002The resulting single-cell suspension was incubated with blocking with 2% fetal calf serum in HBSS containing c-Kit (Abcam, ab25022) at 1:200 dilution cells for 30 min on ice and incubated for ...

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  • Risk for the development of Antimicrobial Resistance

    Colostrum is essential for the health of the newborn calf. Calves are born agammaglobulinaemic (Godden, 2008). Until they develop their own ability to resist disease, through exposure to the microorganisms in their surroundings, they depend entirely on the immunity acquired by the consumption of colostrum (Moran, 2016). First colostrum contains ...

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  • Hydrogel‐Forming Microneedle Arrays for Enhanced ...

    2012-7-9u2002·u2002The receptor compartment was filled with 12.0 ml of DMEM (Dulbecco's modified Eagle's medium) containing 4.5 g L −1 glucose, 2.0 mM L-glutamine, 10% foetal calf serum, 1.0 IU ml −1 penicillin and 1.0 μg ml −1 streptomycin. After 24 h of application, …

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  • Small rat islets are superior to large islets in in vitro ...

    2006-5-1u2002·u2002After incubation, the tube was gently shaken to dislodge islets. The contents of the tube were placed in diluted ice-cold Hank's balanced salt solution (HBSS) containing 10% of newborn calf serum. The digest was allowed to settle at 1 g, and the supernatant was removed. More HBSS serum was added and the process repeated.

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  • Conditioned medium produced by fibroblasts cultured in

    2020-6-9u2002·u2002HUVECs were centrifuged and resuspended in M199 medium supplemented with 20% newborn calf serum (NCS), 2.28 mM glutamine (Invitrogen), 40 IU/mL heparin (Leo Laboratories, Pickering, Canada), 20 mg ...

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  • Non-invasive detection of iron deficiency by fluorescence ...

    2016-2-17u2002·u2002Worldwide, more individuals have iron deficiency than any other health problem. Most of those affected are unaware of their lack of iron, in part because detection of iron deficiency has required ...

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  • Camel Milk-Clotting Using Plant Extracts as a Substitute ...

    2021-3-5u2002·u2002The transformation of camel milk into cheese is an operation considered very delicate because of several difficulties encountered in achieving coagulation. The present study aims to improve coagulation abilities of camel milk using enzyme extracts from pineapple, kiwi, and ginger. Our results concerning the characterization of the enzymatic extract showed an extraction yield that varies ...

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  • Spa Equipment - Pure Spa Direct

    Spa Equipment... Offer world-class services and treatments to set your spa apart from the competition by upgrading your equipment today! Pure Spa Direct offers an enormous selction of equipment, from tried-and-true modalities like high frequency, galvanic, and ultrasound to cutting-edge new prcedures, including hydroderm abrasion and radio frequency.

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  • ERYTHROPOIETIN: ELUCIDATING NEW CELLULAR

    2. Structural and Molecular Determinants of EPO Activity. EPO is a 30.4 kDa glycoprotein with approximately half of its molecular weight derived from carbohydrates that can vary among species (Maiese et al., 2005c).EPO contains four glycosylated chains including three N-linked and one O-linked acidic oligosaccharide side chains.N-linked glycosylation sites occur at the positions 24, 38, and 83 ...

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  • Dairy Test Bank Flashcards

    In 2004 nearly ________ of total US farm milk production ended up in exported US dairy products. 7.50%. Cheese imports account for _______ of the total value of US dairy imports. 42%. The total volume of US dairy exports rose 38.5% in 2004 with most of the increase driven by …

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