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2020-11-9u2002·u2002THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1987 by The American Society of Biological Chemists, Inc. Vol. 262, No. 1, Issue of January 5, pp. 123-129,1987 Printed in U.S.A. The Interaction of Phosphorylated Oligosaccharides and Lysosomal Enzymes with Bovine Liver Cation-dependent Mannose 6-Phosphate Receptor* (Received for publication, July 21, 1986)
Get Price1987-1-5u2002·u2002THE JOURNALBIOLOGICAL OF CHEMISTRY 0 1987 by The American Society of Biological Chemists, Inc. Vol. 262, No. 1, Issue of January 5, pp. 123-129,1987 Printed i U.S.A. n The Interactionof Phosphorylated Oligosaccharides and Lysosomal Enzymes with Bovine Liver Cation-dependentMannose 6-Phosphate Receptor* (Received for publication, July 21, 1986) Bernard HoflackS, Karen Fujimoto, and ...
Get Price2021-9-7u2002·u2002The cell pellets were resuspended in 20 mL growth media, filtered through a 40-μm cell strainer, centrifuged at 600 xg for 5 min, resuspended in 10 mL growth media, and plated in 10-cm collagen-coated dishes. The cells were cultured in growth media (DMEM/F-12 Glutamax, Thermo Fisher Scientific #10565018) supplemented with 10% fetal bovine serum.
Get Price2019-5-15u2002·u2002The current study is the first to characterize the plasma membrane localization of OATP1B1 using the c.521 T > C and c. 388A > G OATP1B1 genotyped liver tissues and provide direct evidence showing that the V174A-OATP1B1 has predominant plasma membrane localization on the basolateral membrane of hepatocytes, similar as the WT-OATP1B1 (Fig. 1 A j-l).
Get Price2007-1-1u2002·u2002Methods and Results. We used six human hepatoma cell lines, three of which are known Fas-expressing cells. Growth of Fas-positive hepatoma cell lines was inhibited by an agonistic anti-Fas antibody in a dose-dependent manner, and these effects were enhanced by IFNα or 5-FU alone, but even more so by combination therapy using both agents.
Get Price2011-6-5u2002·u2002by Bergstrand and Czar in 1956.1 Alpha-fetoprotein is a single polypeptide chain glycoprotein with a molecular weight of approximately 70,000 daltons. The physicochemical properties and amino acid composition are similar to those of albumin.2,3 Synthesis of AFP occurs primarily in the liver and yolk sac of the fetus.
Get PricePDF Beta-glucuronidase is a lysosomal enzyme that plays an essential role in normal turnover of glycosaminoglycans and remodeling of the extracellular... Find, read and cite all the research ...
Get Price2021-1-1u2002·u2002Tricine SDS-PAGE showed that both 30-kDa MWCO filter and C8 SPE are very effective in enriching for proteins/peptides in the molecular weight range between 5 and 15 kDa (Fig. 2A).LC–MS/MS data further confirmed these results by showing that 12.6% and 13.2% of the total identified annotated proteins in the 30-kDa MWCO filter and C8 SPE approaches, respectively, were low molecular weight ...
Get Price1999-8-1u2002·u2002Comparison of enzyme analyses and direct DNA sequencing, depicted in figure 1, showed very good agreement for 11 of 12 family members from the maternal side of the family of patient 1. Although the results of enzyme studies for the maternal great-grandfather were unclear, direct sequence analysis verified that he carried the I268T allele.
Get PriceThe results of the study showed that the total percentage of infection of bovine respiratory syncytial virus was 83.11%, 30.89%, 37.3% using indirect ELISA, direct ELISA, and Nested RT-PCR ...
Get Price2021-9-17u2002·u2002Drug and target discovery for advanced liver disease are hampered by a lack of suitable models for clinical translation. Here the authors present a human liver cell-based system modeling a ...
Get Price2019-6-25u2002·u2002The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential ... direct trans-differentiation of fibroblasts to hepato-cytes, circumventing the pluripotent state.(5-7) iPSCs ... replacer (Gibco),5% fetal bovine serum (FBS),10 ng/mL (BD). Cells were either acquired on a Gallios or
Get Price1991-7-25u2002·u2002T H E J O U R N A L OF BIOLOGICAL C H E M I S T R Y (r‘ 1991 by The American Society for Biochemistry and Molecular Biology, Inc. Vol. 266, No. 21, Issue of July 25, pp. 14082-14087,1991 Printed in U. S. A. Cloning and Expression of a Pharmacologically Unique Bovine Peripheral-type Benzodiazepine Receptor Isoquinoline Binding Protein* (Received for publication, January 14, …
Get Price2018-12-5u2002·u2002Uptake of 3 H-T by LAPC-4 and VCaP cells was evaluated over a time course of 60 min (Fig. 1A). Cells were treated with 1 nM 3 H-T. LAPC-4 and VCaP cells showed similar uptake patterns. The 3 H-T accumulation was nearly linear over the time course. Therefore, 20 min treatment duration was used for dynamic experiments for sufficient scintillation count readings and the shortest treatment ...
Get PriceA, The releasing profile of 20 mg oxaliplatin-loaded CSM with size 50 to 150 mm, 100 to 300 mm, and 300 to 500 mm. B, The effect of FBS on releasing profiles of oxaliplatin-loaded CSM.
Get PriceExtracts from fetal bovine tendon were resolved by gel electrophoresis and electrophoretically transferred to nitrocellulose. ... France. %I.: 33-72-72-26-67; ... direct interactions between the ...
Get Price2021-3-8u2002·u2002Cancer is a major cause of death with more than 1.8 million new cases estimated to be diagnosed in 2020 in the United States alone 1.Cancer immunotherapy has …
Get PriceOccludin is a protein component of the membrane domain of tight junctions, and has been shown to be phosphorylated in vivo in cultured cells and Xenopus laevis embryos. However, nothing is known abou...
Get Price2019-7-2u2002·u2002EGF, BTC, IGF-I, IGF-II, TGF-beta 1, TGF-beta 2, FGF1 and 2, and PDGF are the main growth factors present in bovine milk and colostrum. All of …
Get Price1. A fusion polypeptide comprising at least one of each of: a) a human interleukin 2 (IL-2) polypeptide [A]; b) a half-life extension element [B]; c) an IL-2 blocking moiety [D]; and d) a protease-cleavable polypeptide linker [L]; and wherein the IL-2 polypeptide and the IL-2 blocking moiety are operably linked by the protease-cleavable polypeptide linker and the fusion polypeptide has ...
Get Price2021-9-17u2002·u2002Drug and target discovery for advanced liver disease are hampered by a lack of suitable models for clinical translation. Here the authors present a human liver cell-based system modeling a ...
Get Price2020-4-10u2002·u2002Hepatocyte-like cells (iHEPs) generated by transcription factor-mediated direct reprogramming of somatic cells have been studied as potential cell sources for the development of novel therapies targeting liver diseases. The mechanisms involved in direct reprogramming, stability after long-term in vitro expansion, and safety profile of reprogrammed cells in different experimental models ...
Get PriceExtracts from fetal bovine tendon were resolved by gel electrophoresis and electrophoretically transferred to nitrocellulose. ... France. %I.: 33-72-72-26-67; ... direct interactions between the ...
Get PriceA, The releasing profile of 20 mg oxaliplatin-loaded CSM with size 50 to 150 mm, 100 to 300 mm, and 300 to 500 mm. B, The effect of FBS on releasing profiles of oxaliplatin-loaded CSM.
Get Price2019-7-2u2002·u2002EGF, BTC, IGF-I, IGF-II, TGF-beta 1, TGF-beta 2, FGF1 and 2, and PDGF are the main growth factors present in bovine milk and colostrum. All of …
Get PriceOccludin is a protein component of the membrane domain of tight junctions, and has been shown to be phosphorylated in vivo in cultured cells and Xenopus laevis embryos. However, nothing is known ab...
Get Price2018-12-5u2002·u2002The hormone insulin plays a central role in the metabolism of carbohydrates, lipids, and proteins. In relation to protein metabolism, insulin stimulates amino acid uptake and activates protein synthesis in responsive cells by modulation of signal transduction pathways, such as associated to Akt/PkB, mTOR, S6Ks, 4E-BP1, and several translation initiation/elongation factors. In this context ...
Get PriceThe fetus then produces its own insulin resulting in hyperinsulinemia. High levels of insulin combined with high levels of glucose in the fetus result in storage of excess fat in the abdomen of the fetus leading to macrosomia. 18, 19 The third trimester is the time of rapid weight gain, development of muscle mass and fat stores for the newborn.
Get PriceCalcitonin is a polypeptide secreted from parafollicular C cells of the thyroid. It inhibits bone resorption by its direct action on the osteoclast calcitonin receptor [ 50 ] . Its quick action leads to loss of ruffled border of osteoclasts and decreased number of osteoclasts.
Get PriceMoreira and Sales stated that it would be best for people with diabetic foot diseases to perform their own care. Nurses should educate the patient and then direct the patient rather than taking control of the care. Similarly, Ren et al. investigated the importance of nursing education in high-risk diabetic foot patients. In the two-year follow ...
Get Price2004-6-16u2002·u2002Listeria monocytogenes is a Gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. Signature-tagged mutagenesis (STM) was used to identify a gene named fbpA, required for efficient liver colonization of mice inoculated intravenously.FbpA was also shown to be required for intestinal and liver colonization after oral infection of transgenic ...
Get Price2018-1-18u2002·u2002The NALP3 inflammasome interacts with various immune and cell metabolic pathways and may participate in pulmonary fibrosis. However, little is known on its regulatory mechanism with respect to collagen synthesis. The objective of the present study was to investigate whether NALP3 inflammasome activation is involved in H2O2‑mediated collagen synthesis, in addition to examining the possible ...
Get Price2001-2-21u2002·u2002Serial dilutions of BALF showed good linearity, and excellent recovery rates were obtained upon spiking of human BALF. A mean value of 579.5 ± 45.9 ng/ml (mean ± SEM) SP-C was found in BALF samples of human healthy volunteers (n = 22), corresponding to 26.61 ± 1.91 μ g SP-C/mg total phospholipids (PL).
Get Price1. A fusion polypeptide comprising at least one of each of: a) a human interleukin 2 (IL-2) polypeptide [A]; b) a half-life extension element [B]; c) an IL-2 blocking moiety [D]; and d) a protease-cleavable polypeptide linker [L]; and wherein the IL-2 polypeptide and the IL-2 blocking moiety are operably linked by the protease-cleavable polypeptide linker and the fusion polypeptide has ...
Get PriceReprogramming of somatic cells into induced pluripotent stem cells (iPS) or directly into cells from a different lineage, including neurons, has revolutionized research in regenerative medicine in recent years. Mesenchymal stem cells are good candidates for lineage reprogramming and autologous transplantation, since they can be easily isolated from accessible sources in adult humans, such as ...
Get Price2016-6-7u2002·u20021 About 39,230 new cases of liver cancer (28,410 in men and 10,820 in women) were predicted in 2016 in the USA. 2 Hepatocellular carcinoma (HCC) accounting for over 90% of primary liver cancer, mainly occurs in Asia and Africa, while its incidence is steadily increased in United States over the past 30 years. 3 In addition to the chronic liver injury ...
Get PriceOccludin is a protein component of the membrane domain of tight junctions, and has been shown to be phosphorylated in vivo in cultured cells and Xenopus laevis embryos. However, nothing is known abou...
Get Price2012-6-1u2002·u2002Most of the liver resections were the result of metastatic colon cancer. Organs not used for orthotopic liver transplantation were obtained 3–24 h after cross-clamp and in situ perfusion. Two of the donor livers were from non-heart-beating donors and were reported to …
Get PriceThen, you describe the specific details of the paper you need: add the topic, write or paste the instructions, and attach files to be used, if you have them. After that, an online customer support representative chooses the best writer that specializes in your discipline, and assigns him or her to complete the paper according to your requirements.
Get PriceExample: Drink 5 to 10 oz of water. peri exercise strength athlete. Carbohydrate intake (and possibly protein) should begin shortly after onset of activity only if exercise session lasts more than 60 minutes. 30 to 60 g carbohydrates per hour spaced every 15 to 20 minutes for exercise lasting over an hour.
Get Price2020-11-9u2002·u2002THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1987 by The American Society of Biological Chemists, Inc. Vol. 262, No. 1, Issue of January 5, pp. 123-129,1987 Printed in U.S.A. The Interaction of Phosphorylated Oligosaccharides and Lysosomal Enzymes with Bovine Liver Cation-dependent Mannose 6-Phosphate Receptor* (Received for publication, July 21, 1986)
Get Price1987-1-5u2002·u2002THE JOURNALBIOLOGICAL OF CHEMISTRY 0 1987 by The American Society of Biological Chemists, Inc. Vol. 262, No. 1, Issue of January 5, pp. 123-129,1987 Printed i U.S.A. n The Interactionof Phosphorylated Oligosaccharides and Lysosomal Enzymes with Bovine Liver Cation-dependentMannose 6-Phosphate Receptor* (Received for publication, July 21, 1986) Bernard HoflackS, Karen Fujimoto, …
Get Price2021-9-7u2002·u2002The cell pellets were resuspended in 20 mL growth media, filtered through a 40-μm cell strainer, centrifuged at 600 xg for 5 min, resuspended in 10 mL growth media, and plated in 10-cm collagen-coated dishes. The cells were cultured in growth media (DMEM/F-12 Glutamax, Thermo Fisher Scientific #10565018) supplemented with 10% fetal bovine serum.
Get Price2019-5-15u2002·u2002The current study is the first to characterize the plasma membrane localization of OATP1B1 using the c.521 T > C and c. 388A > G OATP1B1 genotyped liver tissues and provide direct evidence showing that the V174A-OATP1B1 has predominant plasma membrane localization on the basolateral membrane of hepatocytes, similar as the WT-OATP1B1 (Fig. 1 A j-l).
Get Price2007-1-1u2002·u2002Methods and Results. We used six human hepatoma cell lines, three of which are known Fas-expressing cells. Growth of Fas-positive hepatoma cell lines was inhibited by an agonistic anti-Fas antibody in a dose-dependent manner, and these effects were enhanced by IFNα or 5-FU alone, but even more so by combination therapy using both agents.
Get Price2011-6-5u2002·u2002by Bergstrand and Czar in 1956.1 Alpha-fetoprotein is a single polypeptide chain glycoprotein with a molecular weight of approximately 70,000 daltons. The physicochemical properties and amino acid composition are similar to those of albumin.2,3 Synthesis of …
Get PricePDF Beta-glucuronidase is a lysosomal enzyme that plays an essential role in normal turnover of glycosaminoglycans and remodeling of the extracellular... Find, read and cite all the research ...
Get Price2021-1-1u2002·u2002Tricine SDS-PAGE showed that both 30-kDa MWCO filter and C8 SPE are very effective in enriching for proteins/peptides in the molecular weight range between 5 and 15 kDa (Fig. 2A).LC–MS/MS data further confirmed these results by showing that 12.6% and 13.2% of the total identified annotated proteins in the 30-kDa MWCO filter and C8 SPE approaches, respectively, were low molecular weight ...
Get Price1999-8-1u2002·u2002Comparison of enzyme analyses and direct DNA sequencing, depicted in figure 1, showed very good agreement for 11 of 12 family members from the maternal side of the family of patient 1. Although the results of enzyme studies for the maternal great-grandfather were unclear, direct sequence analysis verified that he carried the I268T allele.
Get PriceThe results of the study showed that the total percentage of infection of bovine respiratory syncytial virus was 83.11%, 30.89%, 37.3% using indirect ELISA, direct ELISA, and Nested RT-PCR ...
Get Price2006-12-1u2002·u2002early embryonic mortality is a recognized cause of reproductive failure in cattle leading to the loss of a large number of potential calves, retarded genetic progress, and significant loss of money and time in rebreeding cows (38, 49).Mortality results either because of intrinsic defects within the embryo, an inadequate maternal environment, asynchrony between embryo and mother, or failure of ...
Get Price1999-2-16u2002·u2002L929 cells (ATTC CCL-1) and NR6P cells were grown in DMEM medium containing 10% fetal bovine serum at 37°C in 5% CO 2. For experiments involving treatments with sodium molybdate, cells were allowed to reach confluence before supplementing the growth medium to a final concentration of 1 mM sodium molybdate, and cultivation of the cells was ...
Get PriceSirtuin 6 (SIRT6), a member of the mammalian sirtuin family, is a nuclear deacetylase with substrate-specific NAD+-dependent activity. SIRT6 has emerged as a critical regulator of diverse processes, including DNA repair, gene expression, telomere maintenance, and metabolism. However, our knowledge regarding its interactions and regulation remains limited.
Get Price2012-11-13u2002·u2002Liver diseases related to the human hepatitis B virus (HBV) kill about 1 million people every year, and more than 350 million people around the world are infected with the virus. Some 15 million of these people are also infected with the hepatitis D virus (HDV), which is a satellite virus of HBV, and this places them at an even higher risk of liver diseases, including cancer.
Get PriceImaging reporter genes provides longitudinal information on the biodistribution, growth, and survival of engineered cells in vivo. A translational bottleneck to using reporter genes is the necessity to engineer cells with randomly integrating vectors. Here, we built homology-independent targeted integration (HITI) CRISPR-Cas9 minicircle donors for precise safe harbor-targeted knock-in of ...
Get Price2012-9-13u2002·u2002The cells were maintained in DMEM or Lonza Xpress medium respectively, supplemented with 10% fetal bovine serum (HyClone), penicillin (200 U/ml) and streptomycin (200 mg/ml). For the induction of stress response, cells were treated with H 2 O 2 (10 and 100 μM), sorbitol (0.5 M) and rotenone (50 μM) for 30 min.
Get Price2016-7-1u2002·u2002Stress at labor is associated with high glucocorticoid signaling (Barlow et al., 1974).We previously reported that glucocorticoids stimulate PPARα expression in the adult liver, but the mechanism was not elucidated (Lemberger et al., 1994).Interestingly, the mRNA expression of GR (Nr3c1) in the fetal liver is the highest just before birth (Speirs et al., 2004).Because GR plays a role in the ...
Get Price2021-6-30u2002·u2002The L, M, and S GPs of HBV are produced by a single open reading frame and share a common C-terminal S domain. M and L proteins harbor additional N-terminal extensions (preS2 and preS1/preS2, respectively), with preS1 harboring the NTCP-binding determinant (Glebe et al., 2005; Gripon et al., 2005).Noteworthy, the fusion determinants of HBV GPs and, particularly, the fusion …
Get Price2019-6-2u2002·u2002Human hepatoma cell lines (HepG2 and Huh7), hepatic stellate cell line LX-2 and human pancreatic cancer MIAPaCa-2 cells were maintained in Roswell Park Memorial Institute medium (RPMI 1640) (Sigma, St. Louis, MO, USA) supplemented with 1-10% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 5% CO2 and 37°C.
Get PriceThe invention claimed is: 1. A method of preparing a heteromultimer comprising a first polypeptide and a second polypeptide each comprising a C H 3 antibody constant domain, wherein said polypeptides meet at an engineered interface within the C H 3 domain, the method comprising the steps of: (a) creating an engineered protuberance in the interface of the first polypeptide by replacing at least ...
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